ArticleProceedings of the National Academy of Sciences of the United States of America2023
Cooperative action of separate interaction domains promotes high-affinity DNA binding of
Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Nanoclustering of a plant transcription factor enables strong yet specific DNA binding.Science advances · 2026Article
- Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies.FEBS open bio · 2026Article
- The (un)likelihood of clock-driven lateral root priming; a modeling exploration.The Plant cell · 2026Article
- A revised model for PHF20L1 Tudor function: DNA binding overrides methylation selectivity on nucleosomes.The Journal of biological chemistry · 2026Article
- The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylation.Nucleic acids research · 2025Article
- Transcription Factors in Plant Gene Expression Regulation.International journal of molecular sciences · 2025Article
- A conserved ARF-DNA interface underlies auxin-triggered transcriptional response.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Cytosine Methylation Changes the Preferred Cis-Regulatory Configuration of Arabidopsis WUSCHEL-Related Homeobox 14.International journal of molecular sciences · 2025Article
- Genome-Wide Identification, Expression, and Interaction Analysis of the Auxin Response Factor andInternational journal of molecular sciences · 2024Article
- Game of thrones among AUXIN RESPONSE FACTORs-over 30 years of MONOPTEROS research.Journal of experimental botany · 2023Review
- To bind or not to bind: how AUXIN RESPONSE FACTORs select their target genes.Journal of experimental botany · 2023Review
- Clade-D auxin response factors regulate auxin signaling and development in the moss Physcomitrium patens.PLoS biology · 2023Article
- Transcriptional Tuning: How Auxin Strikes Unique Chords in Gene Regulation.Physiologia plantarumReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The signaling molecule auxin coordinates many growth and development processes in plants, mainly through modulating gene expression. Transcriptional response is mediated by the family of auxin response factors (ARF). Monomers of this family recognize a DNA motif and can homodimerize through their DNA-binding domain (DBD), enabling cooperative binding to an inverted binding site. Most ARFs further contain a C-terminal PB1 domain that is capable of homotypic interactions and mediating interactions with Aux/IAA repressors. Given the dual role of the PB1 domain, and the ability of both DBD and PB1 domain to mediate dimerization, a key question is how these domains contribute to DNA-binding specificity and affinity. So far, ARF-ARF and ARF-DNA interactions have mostly been approached using qualitative methods that do not provide a quantitative and dynamic view on the binding equilibria. Here, we utilize a DNA binding assay based on single-molecule Förster resonance energy transfer (smFRET) to study the affinity and kinetics of the interaction of several
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