Evidence map›Paper›PMID 36881305›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Live Imaging of Axonal Dynamics After Laser Axotomy of Peripheral Neurons in Zebrafish.

Kadidia P Adula, Alvaro Sagasti

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 93% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Kadidia P AdulaDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA.
Alvaro SagastiDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA. sagasti@mcdb.ucla.edu.
University of California, Los Angeles · US

Funding

Peripheral Territory Establishment By Zebrafish Somatosensory NeuronsR01AR064582 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SAGASTI, ALVARO · 2014 to 2024
$3.6M
Do somatosensory endings use axonal or dendritic regeneration pathways?R21NS090027 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROLLS, MELISSA, SAGASTI, ALVARO · 2014 to 2015
$416k
Intrinsic Pathways Regulating Sensory Axon Regeneration in Zebrafish LarvaeF31NS106742 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ADULA, KADIDIA PEMBA · 2018 to 2020
$105k
NIAMS NIH HHS R01 AR064582NINDS NIH HHS F31 NS106742NINDS NIH HHS R21 NS090027
6 · The paper itself

Abstract

Axon severing results in diverse outcomes, including successful regeneration and reestablishment of function, failure to regenerate, or neuronal cell death. Experimentally injuring an axon makes it possible to study degeneration of the distal stump that was detached from the cell body and document the successive steps of regeneration. Precise injury reduces damage to the environment surrounding an axon, and thereby the involvement of extrinsic processes, such as scarring or inflammation, enabling researchers to isolate the role that intrinsic factors play in regeneration. Several methods have been used to sever axons, each with advantages and disadvantages. This chapter describes using a laser on a two-photon microscope to cut individual axons of touch-sensing neurons in zebrafish larvae, and live confocal imaging to monitor its regeneration, a method that provides exceptional resolution.

Indexed as

AxonsZebrafishAnimalsAxotomyLasersNeuronsAxonAxotomyLive imagingRegenerationSomatosensoryTransient transgenesisZebrafish

Identifiers

PMID36881305
OpenAlexW4323347390

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.