ArticleAmerican journal of physiology. Cell physiology2023
Physiologically relevant culture medium Plasmax improves human placental trophoblast stem cell function.
Article in American journal of physiology. Cell physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 5 citations in OpenAlex.
- 3D Ewing Sarcoma Modeling for Metabolic Studies.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Isthmin-2 is a first-trimester predictor of preeclampsia and fetal growth restriction.Nature medicine · 2026Article
- Applying the Lessons of Physiological Cell Culture to Human Embryo Culture for In Vitro Fertilization.Biomolecules · 2026Review
- Extravillous trophoblasts reverse the decidualization induced increase in matrix production by secreting TGFβ antagonists Emilin-1 and Gremlin-1.Cells & development · 2025Article
- Effect of Media Composition and Oxygen Tension on Cellular Stress Response and Nrf2 Activation in HepG2ARE Cells.Antioxidants (Basel, Switzerland) · 2025Article
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Authors and funding
7 authors at 2 institutions in 1 country.
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Abstract
Human trophoblast cultures provide powerful tools to model key processes of placental development. In vitro trophoblast studies to date have relied on commercial media that contains nonphysiological levels of nutrients, and the impact of these conditions on trophoblast metabolism and function is unknown. Here, we show that the physiological medium (Plasmax) with nutrient and metabolite concentrations recapitulating human plasma improves human trophoblast stem cell (hTSC) proliferation and differentiation compared with standard medium (DMEM-F12). hTSCs cultured in Plasmax-based medium also show altered glycolytic and mitochondrial metabolism, as well as reduced S-adenosylmethionine/S-adenosyl-homocysteine ratio compared with DMEM-F12-based medium. These findings demonstrate the importance of the nutritional environment for phenotyping cultured human trophoblasts.
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Registered trials
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