ArticleActa crystallographica. Section D, Structural biology2023
20 years of crystal hits: progress and promise in ultrahigh-throughput crystallization screening.
Article in Acta crystallographica. Section D, Structural biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- AXIS: a Lab-in-the-Loop machine learning approach for automated detection of macromolecular crystals.IUCrJ · 2026Article
- Structural and oligomeric characterization of substrate- and product-selective nylon hydrolases.bioRxiv : the preprint server for biology · 2026Article
- Structure of the disulfide-rich modules of a striking tandem repeat protein, avian cysteine-rich eggshell membrane protein.Protein science : a publication of the Protein Society · 2026Article
- Preparing for successful protein crystallization experiments.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Article
- A generic cross-seeding approach to protein crystallization.Journal of applied crystallography · 2025Article
- Identification and characterization of substrate- and product-selective nylon hydrolases.bioRxiv : the preprint server for biology · 2024Article
- Managing macromolecular crystallographic data with a laboratory information management system.Acta crystallographica. Section D, Structural biology · 2024Article
- Reaching the potential of electron diffraction.Cell reports. Physical science · 2024Article
- Applying 3D ED/MicroED workflows toward the next frontiers.Acta crystallographica. Section C, Structural chemistry · 2024Article
- X-ray crystal structure of a designed rigidified imaging scaffold in the ligand-free conformation.Acta crystallographica. Section F, Structural biology communications · 2024Article
- The structure of the monobactam-producing thioesterase domain of SulM forms a unique complex with the upstream carrier protein domain.bioRxiv : the preprint server for biology · 2024Article
- Systematic enhancement of protein crystallization efficiency by bulk lysine-to-arginine (KR) substitution.Protein science : a publication of the Protein Society · 2024Article
- High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography.Journal of visualized experiments : JoVE · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Diffraction-based structural methods contribute a large fraction of the biomolecular structural models available, providing a critical understanding of macromolecular architecture. These methods require crystallization of the target molecule, which remains a primary bottleneck in crystal-based structure determination. The National High-Throughput Crystallization Center at Hauptman-Woodward Medical Research Institute has focused on overcoming obstacles to crystallization through a combination of robotics-enabled high-throughput screening and advanced imaging to increase the success of finding crystallization conditions. This paper will describe the lessons learned from over 20 years of operation of our high-throughput crystallization services. The current experimental pipelines, instrumentation, imaging capabilities and software for image viewing and crystal scoring are detailed. New developments in the field and opportunities for further improvements in biomolecular crystallization are reflected on.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.