ArticlePeerJ2023
Addressing the challenges of symbiont-mediated RNAi in aphids.
Article in PeerJ, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- Delivery, Persistence, and Functional Response of dsRNA in Acyrthosiphon pisum: Progress and Remaining Barriers.Archives of insect biochemistry and physiology · 2026Article
- Can the leafhopper microbiome unlock new strategies for its control?Journal of economic entomology · 2026Review
- Genetic Biosensor for Optimizing Double-Stranded RNA Production by Bacteria.ACS synthetic biology · 2026Article
- Aphid adaptation to plant secondary metabolites: adaptive mechanism of resistance evolution and future prospects.Horticulture research · 2026Article
- Ecological Mercenaries: Why Aphids Remain Premier Models for the Study of Ecological Symbiosis.Insects · 2025Review
- An ancient immune pathway enables animal metamorphosis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Review
- The intestinal microbial community and function ofFrontiers in microbiology · 2025Article
- Exploring the challenges of RNAi-based strategies for crop protection.Advanced biotechnology · 2024Review
- The Genome ofInsects · 2024Article
- Improving RNA-based crop protection through nanotechnology and insights from cross-kingdom RNA trafficking.Current opinion in plant biology · 2023Review
- The pivotal roles of gut microbiota in insect plant interactions for sustainable pest management.NPJ biofilms and microbiomes · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Because aphids are global agricultural pests and models for bacterial endosymbiosis, there is a need for reliable methods to study and control their gene function. However, current methods available for aphid gene knockout and knockdown of gene expression are often unreliable and time consuming. Techniques like CRISPR-Cas genome editing can take several months to achieve a single gene knockout because they rely on aphids going through a cycle of sexual reproduction, and aphids often lack strong, consistent levels of knockdown when fed or injected with molecules that induce an RNA interference (RNAi) response. In the hopes of addressing these challenges, we attempted to adapt a new method called symbiont-mediated RNAi (smRNAi) for use in aphids. smRNAi involves engineering a bacterial symbiont of the insect to continuously supply double-stranded RNA (dsRNA) inside the insect body. This approach has been successful in thrips, kissing bugs, and honeybees. We engineered the laboratory
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.