Evidence map›Paper›PMID 36874158›Full record

ArticleComputational and structural biotechnology journal2023

Short-chain fatty acids reprogram metabolic profiles with the induction of reactive oxygen species production in human colorectal adenocarcinoma cells.

Chongyang Huang, Wenjun Deng, Huan-Zhou Xu, Chen Zhou, Fan Zhang, Junfei Chen, Qinjia Bao, Xin Zhou, Maili Liu, Jing Li and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Computational and structural biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 29 citations in OpenAlex.

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  16. Antioxidant Role of Probiotics in Inflammation-Induced Colorectal Cancer.International journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Chongyang HuangState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, China.
Wenjun DengWuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.
Huan-Zhou XuDepartment of Pediatrics, Division of Infectious Diseases, College of Medicine, University of Florida, Gainesville, FL, USA.
Chen ZhouWuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.
Fan ZhangWuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
Junfei ChenState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, China.
Qinjia BaoState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, China.
Xin ZhouState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, China.
Maili LiuState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, China.
Jing LiWuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.
Chaoyang LiuState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, China.
Chinese Academy of Sciences · CNWuhan Botanical Garden · CNUniversity of Florida · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Short-chain fatty acids (SCFAs) exhibit anticancer activity in cellular and animal models of colon cancer. Acetate, propionate, and butyrate are the three major SCFAs produced from dietary fiber by gut microbiota fermentation and have beneficial effects on human health. Most previous studies on the antitumor mechanisms of SCFAs have focused on specific metabolites or genes involved in antitumor pathways, such as reactive oxygen species (ROS) biosynthesis. In this study, we performed a systematic and unbiased analysis of the effects of acetate, propionate, and butyrate on ROS levels and metabolic and transcriptomic signatures at physiological concentrations in human colorectal adenocarcinoma cells. We observed significantly elevated levels of ROS in the treated cells. Furthermore, significantly regulated signatures were involved in overlapping pathways at metabolic and transcriptomic levels, including ROS response and metabolism, fatty acid transport and metabolism, glucose response and metabolism, mitochondrial transport and respiratory chain complex, one-carbon metabolism, amino acid transport and metabolism, and glutaminolysis, which are directly or indirectly linked to ROS production. Additionally, metabolic and transcriptomic regulation occurred in a SCFAs types-dependent manner, with an increasing degree from acetate to propionate and then to butyrate. This study provides a comprehensive analysis of how SCFAs induce ROS production and modulate metabolic and transcriptomic levels in colon cancer cells, which is vital for understanding the mechanisms of the effects of SCFAs on antitumor activity in colon cancer.

Indexed as

1H–13C HMBC, 1H–13C Heteronuclear Multiple Bond Correlation Spectroscopy1H–13C HSQC, 1H–13C Heteronuclear Single Quantum Coherence Spectroscopy1H–1H COSY, 1H–1H Correlation Spectroscopy1H–1H TOCSY, 1H–1H Total Correlation SpectroscopyAce, AcetateAch, AcetylcholineADP, Adenosine diphosphateAla, AlanineAMP, Adenosine monophosphateATP, Adenosine triphosphateCaco-2, Human Colon AdenocarcinomaCho, CholineCoA, Coenzyme ACRC, Colorectal CancerCre, CreatineDCFH-DA, Dichloro-Dihydro-Fluorescein DiacetatedDNP, dissolution Dynamic Nuclear PolarizationDEGs, Differentially Expressed GenesDMEM, Dulbecco's Modified Eagle MediumDMG, DimethylglycineDNA, Deoxyribonucleic AcidEP, EppendorfFA, FormateFDR, False Discovery RateFru, FructoseFum, Fumaric acidGal-1-P, Galactose-1-phosphateGlc, GlucoseGln, GlutamineGLS, GlutaminaseGlu, GlutamateGly, GlycineGSEA, Gene Set Enrichment AnalysisGSH, GlutathioneHCT116, Human Colorectal Carcinoma Cell LineHEK, Human Embryonic Kidney cellsHis, HistidineHT29, Human Colorectal Adenocarcinoma Cell Line with Epithelial MorphologyIle, IsoleucineJ-Res, J-resolved SpectroscopyLac, LactateLDH, Lactate DehydrogenaseLeu, LeucineLys, LysineMCF-7, Human Breast Cancer Cell Line with EstrogenMCT, Monocarboxylate TransportersMet, MethionineMetS, Metabolic SyndromeMitochondrial functionNAD+, Nicotinamide adenine dinucleotideNAG, N-Acetyl-L-GlutamineNMR-based MetabolomicsNMR, Nuclear Magnetic ResonanceNOESY, Nuclear Overhauser Effect SpectroscopyO-PLS-DA, Orthogonal Projection to the Latent Structures Discriminant AnalysisPA, PantothenatePCA, Principal Component AnalysisPC, PhosphocholinePDC, Pyruvate DecarboxylasePDK, Pyruvate Dehydrogenase KinasePhe, PhenylalaninePKC, Protein Kinase CPPP, Pentose Phosphate PathwayPyr, PyruvateqRT-PCR, Real-Time Quantitative Reverse Transcription Polymerase Chain ReactionReactive oxygen speciesRNA, Ribonucleic AcidROS, Reactive Oxygen SpeciesRPKM, Reads per Kilobase of Transcript per Million Reads MappedSCFAs, Short Chain Fatty AcidsShort-chain fatty acidsSLC, Solute-Carrier GenesSuc, SuccinateT2DM, Type 2 DiabetesTau, TaurineTCA, Tricarboxylic AcidThr, ThreonineTranscriptomicsTyr, TyrosineUDP-GLC, UDP GlucoseUDPGs, UDP Glucose and UDP GlucuronateUDPG, UDP GlucuronateUDP, Uridine 5′-diphosphateUMP, Uridine 5′-monophosphateVal, ValineWST-1, Water-Soluble Tetrazolium saltsα-KIV, α-Keto-isovalerateα-KMV, α-keto-β-methyl-valerate

Identifiers

PMID36874158
PMCPMC9975252
OpenAlexW4320486930

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.