ArticleThe ISME journal2023
Surface-layer protein is a public-good matrix exopolymer for microbial community organisation in environmental anammox biofilms.
Article in The ISME journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 76 citations in OpenAlex.
- Assembly, architecture and functional roles of microbial surface layers.Nature reviews. Microbiology · 2026Review
- Planktonic anammox bacteria toward a better understanding of ecophysiological traits and harnessing the untapped potential as a bioresource.Bioprocess and biosystems engineering · 2026Review
- Stress-induced toxic genomic R-loops support biofilm extracellular matrix formation.Nature communications · 2025Article
- Genomic and Physiological Signatures of Evolution in ANAMMOX Bacteria.Environmental microbiology reports · 2025Review
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- Article
- Mapping the ultrastructural topology of the corynebacterial cell surface.PLoS biology · 2025Article
- Metaproteomic Profiling of the Secretome of a Granule-forming Ca. Accumulibacter Enrichment.Proteomics · 2025Article
- Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genetic Transfer in Action: Uncovering DNA Flow in an Extremophilic Microbial Community.Environmental microbiology · 2025Article
- Microbial extracellular polymeric substances in the environment, technology and medicine.Nature reviews. Microbiology · 2025Review
- Microbial hauberks: composition and function of surface layer proteins in gammaproteobacterial methanotrophs.Applied and environmental microbiology · 2025Article
- Penetrating the biofilm barrier: characterization ofFrontiers in microbiology · 2025Article
- Novel ellipsoid-like granules exhibit enhanced anammox performance compared to sphere-like granules.Water research X · 2024Article
- Mechanisms of microbial co-aggregation in mixed anaerobic cultures.Applied microbiology and biotechnology · 2024Review
- Quest for Nitrous Oxide-reducing Bacteria Present in an Anammox Biofilm Fed with Nitrous Oxide.Microbes and environments · 2024Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular polymeric substances (EPS) are core biofilm components, yet how they mediate interactions within and contribute to the structuring of biofilms is largely unknown, particularly for non-culturable microbial communities that predominate in environmental habitats. To address this knowledge gap, we explored the role of EPS in an anaerobic ammonium oxidation (anammox) biofilm. An extracellular glycoprotein, BROSI_A1236, from an anammox bacterium, formed envelopes around the anammox cells, supporting its identification as a surface (S-) layer protein. However, the S-layer protein also appeared at the edge of the biofilm, in close proximity to the polysaccharide-coated filamentous Chloroflexi bacteria but distal to the anammox bacterial cells. The Chloroflexi bacteria assembled into a cross-linked network at the edge of the granules and surrounding anammox cell clusters, with the S-layer protein occupying the space around the Chloroflexi. The anammox S-layer protein was also abundant at junctions between Chloroflexi cells. Thus, the S-layer protein is likely transported through the matrix as an EPS and also acts as an adhesive to facilitate the assembly of filamentous Chloroflexi into a three-dimensional biofilm lattice. The spatial distribution of the S-layer protein within the mixed species biofilm suggests that it is a "public-good" EPS, which facilitates the assembly of other bacteria into a framework for the benefit of the biofilm community, and enables key syntrophic relationships, including anammox.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.