Evidence map›Paper›PMID 36868498›Full record

ArticleJournal of immunological methods2023

A comprehensive multiparameter flow cytometry panel for immune profiling and functional studies of frozen tissue, bone marrow, and spleen.

Yi-Chu Wu, Michael Kissner, Fatemeh Momen-Heravi

Open access · greenAbstract read
In one paragraph

Article in Journal of immunological methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. OralFrontiers in microbiology · 2026
    Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Yi-Chu WuSection of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University, NY, New York, USA; Cancer Biology and Immunology Laboratory, Columbia University Irving Medical Center, NY, New York, USA.
Michael KissnerColumbia Stem Cell Initiative Flow Cytometry Core, Columbia University Irving Medical Center, NY, New York, USA.
Fatemeh Momen-HeraviSection of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University, NY, New York, USA; Cancer Biology and Immunology Laboratory, Columbia University Irving Medical Center, NY, New York, USA; Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, NY, New York, USA. Electronic address: fm2540@cumc.columbia.edu.
Columbia University · US

Funding

Tumor Biology and Microenvironment ProgramP30CA013696 · NCI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Anil K Rustgi · 1985 to 2026
$115.3M
Clinical and Translational Science AwardUL1TR001873 · NCATS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI REILLY, MUREDACH P · 2016 to 2025
$99.0M
Institutional Career Development CoreKL2TR001874 · NCATS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI GENKINGER, JEANINE M., SHIMBO, DAICHI · 2016 to 2025
$13.6M
Identifying Molecular Subtypes of Head and Neck Cancer in Patients with African AncestryR21DE031112 · NIDCR · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MOMEN HERAVI, FATEMEH, TAYLOR, ALISON M. · 2021 to 2022
$565k
Monocyte and Macrophage Signaling in the Pathogenesis of Periodontitis in DiabetesR03DE029546 · NIDCR · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MOMEN HERAVI, FATEMEH · 2020 to 2021
$324k
NCATS NIH HHS KL2 TR001874NCATS NIH HHS UL1 TR001873NCI NIH HHS P30 CA013696NIDCR NIH HHS L30 DE029070NIDCR NIH HHS R03 DE029546NIDCR NIH HHS R21 DE031112
6 · The paper itself

Abstract

Flow cytometry (FC) is a highly informative technology that can provide valuable information about immune phenotype monitoring and immune cell states. However, there is a paucity of comprehensive panels developed and validated for use on frozen samples. Here, we developed a 17-plex flow cytometry panel to detect subtypes, frequencies, and functions of different immune cells that can be leveraged to study the different cellular characteristics in different disease models, physiological, and pathological conditions. This panel identifies surface markers to characterize T cells (CD8

Indexed as

Bone MarrowSpleenAnimalsBone and BonesFlow CytometryImmunophenotypingMiceMonocytesImmune profilingMulti-color flow cytometryPhenotypic analysisTumor immune cells

Identifiers

PMID36868498
PMCPMC10508641
OpenAlexW4322754463

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.