Evidence map›Paper›PMID 36867944›Full record

ArticleRedox biology2023

Real-time redox adaptations in human airway epithelial cells exposed to isoprene hydroxy hydroperoxide.

Edward R Pennington, Syed Masood, Steven O Simmons, Lisa Dailey, Philip A Bromberg, Rebecca L Rice, Avram Gold, Zhenfa Zhang, Weidong Wu, Yi Yang and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Redox biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 24 citations in OpenAlex.

  1. Strain-Specific Effects ofMicroorganisms · 2026
    Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Edward R PenningtonOak Ridge Institute for Science and Education, Oak Ridge, TN, USA.
Syed MasoodCurriculum in Toxicology and Environmental Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Steven O SimmonsNational Center for Computational Toxicology, U.S. Environmental Protection Agency, Research Triangle Park, NC, USA.
Lisa DaileyPublic Health and Integrated Toxicology Division, U.S. Environmental Protection Agency, Chapel Hill, NC, USA.
Philip A BrombergCenter for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Rebecca L RiceDepartment of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Avram GoldDepartment of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Zhenfa ZhangDepartment of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Weidong WuSchool of Public Health, Xinxiang Medical University, Xinxiang, Henan Province, China.
Yi YangShanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, Shanghai, China.
James M SametPublic Health and Integrated Toxicology Division, U.S. Environmental Protection Agency, Chapel Hill, NC, USA. Electronic address: samet.james@epa.gov.
University of North Carolina at Chapel Hill · USEnvironmental Protection Agency · USEast China University of Science and Technology · CNOak Ridge Associated Universities · USXinxiang Medical University · CN

Funding

Biostatstics for Research in Environmental HealthT32ES007018 · NIEHS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Stephanie Engel, Rebecca Fry · 1985 to 2026
$31.3M
NIEHS NIH HHS T32 ES007018
6 · The paper itself

Abstract

While redox processes play a vital role in maintaining intracellular homeostasis by regulating critical signaling and metabolic pathways, supra-physiological or sustained oxidative stress can lead to adverse responses or cytotoxicity. Inhalation of ambient air pollutants such as particulate matter and secondary organic aerosols (SOA) induces oxidative stress in the respiratory tract through mechanisms that remain poorly understood. We investigated the effect of isoprene hydroxy hydroperoxide (ISOPOOH), an atmospheric oxidation product of vegetation-derived isoprene and a constituent of SOA, on intracellular redox homeostasis in cultured human airway epithelial cells (HAEC). We used high-resolution live cell imaging of HAEC expressing the genetically encoded ratiometric biosensors Grx1-roGFP2, iNAP1, or HyPer, to assess changes in the cytoplasmic ratio of oxidized glutathione to reduced glutathione (GSSG:GSH), and the flux of NADPH and H

Indexed as

GlutathioneHydrogen PeroxideButadienesEpithelial CellsGlucoseGlutathione DisulfideHemiterpenesHumansNADPOxidation-ReductionOxidative StressRespiratory SystemButadienesGlucoseGlutathioneGlutathione DisulfideHemiterpenesHydrogen PeroxideisopreneNADPAir pollutionGlutathioneIsoprene hydroxy hydroperoxideLive cell imagingNADPHOxidative stress

Identifiers

PMID36867944
PMCPMC10011437
OpenAlexW4322011982

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.