Evidence map›Paper›PMID 36866225›Full record

ArticleArXiv2023

A new twist on PIFE: photoisomerisation-related fluorescence enhancement.

Evelyn Ploetz, Benjamin Ambrose, Anders Barth, Richard Börner, Felix Erichson, Achillefs N Kapanidis, Harold D Kim, Marcia Levitus, Timothy M Lohman, Abhishek Mazumder and 5 more

Abstract readPreprint
PubMed Full text at PMC
In one paragraph

Article in ArXiv, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Evelyn PloetzDepartment of Chemistry and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 München, Germany.
Benjamin AmbroseDepartment of Infectious Disease, Faculty of Medicine, Imperial College London, London, W12 0HS, UK, Single Molecule Imaging Group, MRC-London Institute of Medical Sciences, London, W12 0HS, UK.
Anders BarthDepartment of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft 2629 HZ, The Netherlands.
Richard BörnerLaserinstitut Hochschule Mittweida, Mittweida University of Applied Sciences, Mittweida, Germany.
Felix ErichsonLaserinstitut Hochschule Mittweida, Mittweida University of Applied Sciences, Mittweida, Germany.
Achillefs N KapanidisKavli Institute for Nanoscience Discovery, Department of Biological Physics, The University of Oxford, UK.
Harold D KimSchool of Physics, Georgia Institute of Technology, 837 State Street, Atlanta, GA 30332, USA.
Marcia LevitusSchool of Molecular Sciences, Arizona State University, 551 E. University Drive, Tempe, AZ, 85287, USA.
Timothy M LohmanDepartment of Biochemistry and Molecular Biophysics, Washington University in St. Louis School of Medicine, St. Louis, MO 63110, USA.
Abhishek MazumderKavli Institute for Nanoscience Discovery, Department of Biological Physics, The University of Oxford, UK.
David S RuedaDepartment of Infectious Disease, Faculty of Medicine, Imperial College London, London, W12 0HS, UK, Single Molecule Imaging Group, MRC-London Institute of Medical Sciences, London, W12 0HS, UK.
Fabio D SteffenDepartment of Chemistry, University of Zurich, Zurich, Switzerland.
Thorben CordesPhysical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians-Universität München, Großhadernerstr, 2-4, 82152 Planegg-Martinsried, Germany.
Steven W MagennisSchool of Chemistry, University of Glasgow, Joseph Black Building, University Avenue, Glasgow, G12 8QQ, UK.
Eitan LernerDepartment of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Faculty of Mathematics & Science, Edmond J. Safra Campus, Hebrew University of Jerusalem; Jerusalem 9190401, Israel, Center for Nanoscience and Nanotechnology, Hebrew University of Jerusalem; Jerusalem 9190401, Israel.

Funding

Mechanisms of Helicases, Translocases and SSB Proteins involved in Genome MaintenanceR35GM136632 · NIGMS · WASHINGTON UNIVERSITY · PI Timothy M Lohman · 2020 to 2026
$5.5M
Investigation of energetics of sharp DNA bendingR01GM112882 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI KIM, HAROLD D · 2015 to 2023
$2.6M
NIGMS NIH HHS R01 GM112882NIGMS NIH HHS R35 GM136632
6 · The paper itself

Abstract

PIFE was first used as an acronym for protein-induced fluorescence enhancement, which refers to the increase in fluorescence observed upon the interaction of a fluorophore, such as a cyanine, with a protein. This fluorescence enhancement is due to changes in the rate of cis/trans photoisomerisation. It is clear now that this mechanism is generally applicable to interactions with any biomolecule and, in this review, we propose that PIFE is thereby renamed according to its fundamental working principle as photoisomerisation-related fluorescence enhancement, keeping the PIFE acronym intact. We discuss the photochemistry of cyanine fluorophores, the mechanism of PIFE, its advantages and limitations, and recent approaches to turn PIFE into a quantitative assay. We provide an overview of its current applications to different biomolecules and discuss potential future uses, including the study of protein-protein interactions, protein-ligand interactions and conformational changes in biomolecules.

Identifiers

PMID36866225
PMCPMC9980184

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.