Evidence map›Paper›PMID 36865163›Full record

ArticlebioRxiv : the preprint server for biology2023

Mechanism of outer membrane destabilization by global reduction of protein content.

Irina V Mikheyeva, Jiawei Sun, Kerwyn Casey Huang, Thomas J Silhavy

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Irina V MikheyevaDepartment of Molecular Biology, Princeton University, Princeton, NJ 08540, USA.
Jiawei SunDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Kerwyn Casey HuangDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Thomas J SilhavyDepartment of Molecular Biology, Princeton University, Princeton, NJ 08540, USA.
Princeton University · USChan Zuckerberg Initiative (United States) · US

Funding

Biogenesis and maintenance of the outer membrane of Gram-negative bacteriaR35GM118024 · NIGMS · PRINCETON UNIVERSITY · PI SILHAVY, THOMAS J. · 2016 to 2025
$8.1M
Molecular mechanisms of Gram-negative outer membrane organizationF32GM139232 · NIGMS · PRINCETON UNIVERSITY · PI MIKHEYEVA-BRIDGES, IRINA V. · 2020 to 2022
$119k
NIGMS NIH HHS F32 GM139232NIGMS NIH HHS R35 GM118024
6 · The paper itself

Abstract

The outer membrane (OM) of Gram-negative bacteria such as Significance Statement: The outer membrane (OM) is a selective permeability barrier that contributes to the intrinsic antibiotic resistance of Gram-negative bacteria. Biophysical characterization of the roles of the component proteins, lipopolysaccharides, and phospholipids is limited by both the essentiality of the OM and its asymmetrical organization. In this study, we dramatically change OM physiology by limiting the protein content, which requires phospholipid localization to the outer leaflet and thus disrupts OM asymmetry. By characterizing the perturbed OM of various mutants, we provide novel insight into the links among OM composition, OM stiffness, and cell shape regulation. These findings deepen our understanding of bacterial cell envelope biology and provide a platform for further interrogation of OM properties.

Identifiers

PMID36865163
PMCPMC9980000
OpenAlexW4321365745

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.