ArticlePlant methods2023
A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves.
Article in Plant methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 26 citations in OpenAlex.
- Peas in Rouge: Tyrosine Supplementation Enhances RUBY Reporter Visibility inPlants (Basel, Switzerland) · 2025Article
- Seeing Red, Selecting True: RUBY-Reported Seed Marker Streamlines CRISPR-Clean Rice Breeding.Rice (New York, N.Y.) · 2025Article
- Comprehensive analysis of RNA-seq data reveals novel insight in the formation of bamboo embryogenic callus in Bambusa changningensis.BMC plant biology · 2025Article
- Development and Future Prospects of Bamboo Gene Science.International journal of molecular sciences · 2025Review
- Biosynthesis and Regulatory Mechanisms of Plant Flavonoids: A Review.Plants (Basel, Switzerland) · 2025Review
- Stable isotope labelling and gene expression analysis reveal dynamic nitrogen-supply mechanisms for rapid growth of Moso bamboo.Horticulture research · 2025Article
- Unlocking the potential of flavonoid biosynthesis through integrated metabolic engineering.Frontiers in plant science · 2025Review
- A simple and efficient method for betalain quantification inFrontiers in plant science · 2024Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundBamboo is a perennial and renewable biomass forest resource and its leaf flavonoid is an antioxidant for biological and pharmacological research. The established genetic transformation and gene editing systems in bamboo are significantly limited by the dependence on bamboo regeneration capability. The way to improve the flavonoid content in bamboo leaves through biotechnology is still not feasible.
resultsHere, we developed an in-planta, Agrobacterium-mediated gene expression method for exogenous genes via wounding and vacuum in bamboo. We demonstrated that the RUBY served as a reporter efficiently expressed in bamboo leaves and shoots, albeit unable to integrate into the chromosome. We have also developed a gene editing system by creating an in situ mutant of the bamboo violaxanthin de-epoxidase (PeVDE) gene in bamboo leaves, with lower NPQ values under the fluorometer, which can serve as a native reporter for gene editing. Furthermore, the bamboo leaves with increased flavonoid content were achieved by knocking out the cinnamoyl-CoA reductase genes.
conclusionsOur method can be applied for the functional characterization of novel genes in a short time and is helpful for bamboo leaf flavonoid biotechnology breeding in the future.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.