ArticleNature methods2023
Combining long-term circuit mapping and network transcriptomics with SiR-N2c.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Dissecting mammalian cortical circuit development at single-cell resolution using inducible barcoded rabies virus.bioRxiv : the preprint server for biology · 2025Article
- Circuit-specific gene editing for precision modulation of neuronal activity with CRISPR-rabies virus.bioRxiv : the preprint server for biology · 2025Article
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- Development of Cre-dependent retrograde trans-multisynaptic tracer based on pseudorabies virus bartha strain.Molecular brain · 2025Article
- Molecular connectomics reveals a glucagon-like peptide 1-sensitive neural circuit for satiety.Nature metabolism · 2024Article
- Early Transcriptional Changes in Feline Herpesvirus-1-Infected Crandell-Rees Feline Kidney Cells.Veterinary sciences · 2024Article
- Article
- Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses.Nature neuroscience · 2024Article
- Third-generation rabies viral vectors have low toxicity and improved efficiency as retrograde labeling tools.Cell reports methods · 2023Article
- Genomic stability of self-inactivating rabies.eLife · 2023Article
- Collicular circuits supporting the perceptual, motor and cognitive demands of ethological environments.Current opinion in neurobiology · 2023Review
- The organization of spinal neurons: Insights from single cell sequencing.Current opinion in neurobiology · 2023Review
- Linking connectome with transcriptome using a self-inactivating rabies virus.Nature methods · 2023Article
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8 authors at 3 institutions in 2 countries.
Funding
Abstract
An exciting frontier in circuit neuroscience lies at the intersection between neural network mapping and single-cell genomics. Monosynaptic rabies viruses provide a promising platform for the merger of circuit mapping methods with -omics approaches. However, three key limitations have hindered the extraction of physiologically meaningful gene expression profiles from rabies-mapped circuits: inherent viral cytotoxicity, high viral immunogenicity and virus-induced alteration of cellular transcriptional regulation. These factors alter the transcriptional and translational profiles of infected neurons and their neighboring cells. To overcome these limitations we applied a self-inactivating genomic modification to the less immunogenic rabies strain, CVS-N2c, to generate a self-inactivating CVS-N2c rabies virus (SiR-N2c). SiR-N2c not only eliminates undesired cytotoxic effects but also substantially reduces gene expression alterations in infected neurons and dampens the recruitment of innate and acquired immune responses, thus enabling open-ended interventions on neural networks and their genetic characterization using single-cell genomic approaches.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.