Evidence map›Paper›PMID 36862266›Full record

ArticlePituitary2023

A procedure in mice to obtain intact pituitary-infundibulum-hypothalamus preparations: a method to evaluate the reconstruction of hypothalamohypophyseal system.

Kai Li, Zhiwei Xiong, Mingfeng Zhou, Yichao Ou, Weizhao Li, Guangsen Wu, Mengjie Che, Haodong Gong, Xingqin Wang, Junjie Peng and 4 more

Abstract read
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In one paragraph

Article in Pituitary, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Kai Li *Department of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Zhiwei Xiong *Department of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Mingfeng Zhou *Department of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Yichao OuDepartment of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Weizhao LiDepartment of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Guangsen WuDepartment of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Mengjie CheDepartment of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Haodong GongDepartment of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Xingqin WangDepartment of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Junjie PengDepartment of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Xiaoxuan ZhengDepartment of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
Jiahui LiThe 74th Military Medical Hospital of Chinese People's Liberation Army, Guangzhou, China.
Zhanpeng FengDepartment of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. feng3388836@smu.edu.cn.
Junxiang PengDepartment of Neurosurgery, Institute of Brain Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. pjxlaozong@126.com.
Nanfang Hospital · CNSouthern Medical University · CNChinese People's Liberation Army · CN

Funding

National Natural Science Foundation of China No. 82201516Natural Science Foundation of Guangdong Province 2021A1515011371Natural Science Foundation of Guangdong Province No. 2021A1515110290President Foundation of Nanfang Hospital, Southern Medical University 2019C001Science and Technology Planning Project of Guangzhou No. 202102020977
6 · The paper itself

Abstract

purposeThe histopathological study of brain tissue is a common method in neuroscience. However, efficient procedures to preserve the intact hypothalamic-pituitary brain specimens are not available in mice for histopathological study.

methodWe describe a detailed procedure for obtaining mouse brain with pituitary-hypothalamus continuity. Unlike the traditional methods, we collect the brain via a ventral approach. We cut the intraoccipital synchondrosis, transection the endocranium of pituitary, broke the spheno-occipital synchondrosis, expose the posterior edge of pituitary, separate the trigeminal nerve, then the intact pituitary gland was preserved.

resultWe report an more effective and practical method to obtain continuous hypothalamus -pituitary preparations based on the preserve of leptomeninges. COMPARED WITH THE EXISTING

methodsOur procedure effectively protects the integrity of the fragile infundibulum preventing the pituitary from separating from the hypothalamus. This procedure is more convenient and efficient.

conclusionWe present a convenient and practical procedure to obtain intact hypothalamic-pituitary brain specimens for subsequent histopathological evaluation in mice.

Indexed as

Pituitary DiseasesPituitary Gland, PosteriorAnimalsHypothalamo-Hypophyseal SystemHypothalamusMicePituitary GlandHypothalamohypophyseal systemMicePituitary hormonePituitary stalkRegeneration

Identifiers

PMID36862266
OpenAlexW4322757439

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.