Evidence map›Paper›PMID 36857087›Full record

ReviewChembiochem : a European journal of chemical biology2023

Tracking the Message: Applying Single Molecule Localization Microscopy to Cellular RNA Imaging.

Benoît Arnould, Alexandria L Quillin, Jennifer M Heemstra

Open access · bronzeAbstract readReview
In one paragraph

Review in Chembiochem : a European journal of chemical biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Benoît ArnouldDepartment of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.
Alexandria L QuillinDepartment of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.
Jennifer M HeemstraDepartment of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.ORCID 0000-0002-7691-8526
Washington University in St. Louis · US

Funding

A chemical biology toolbox for RNA post-transcriptional modification and captureR35GM144075 · NIGMS · WASHINGTON UNIVERSITY · PI Jennifer Heemstra · 2022 to 2026
$2.0M
NIGMS NIH HHS R35 GM144075
6 · The paper itself

Abstract

RNA function is increasingly appreciated to be more complex than merely communicating between DNA sequence and protein structure. RNA localization has emerged as a key contributor to the intricate roles RNA plays in the cell, and the link between dysregulated spatiotemporal localization and disease warrants an exploration beyond sequence and structure. However, the tools needed to visualize RNA with precise resolution are lacking in comparison to methods available for studying proteins. In the past decade, many techniques have been developed for imaging RNA, and in parallel super resolution and single-molecule techniques have enabled imaging of single molecules in cells. Of these methods, single molecule localization microscopy (SMLM) has shown significant promise for probing RNA localization. In this review, we highlight current approaches that allow super resolution imaging of specific RNA transcripts and summarize challenges and future opportunities for developing innovative RNA labeling methods that leverage the power of SMLM.

Indexed as

RNASingle Molecule ImagingMicroscopy, FluorescenceRNAFluorscenceNucleic AcidsRNASMLMSuper-Resolution Microscopy

Identifiers

PMID36857087
PMCPMC10192057
OpenAlexW4322720069

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.