Evidence map›Paper›PMID 36856210›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2023

[Silenced ANP32A inhibits the growth, invasion and migration of colorectal cancer

H Ding, X Li, L Zhou, Z Cui, H Meng, J Wang

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Role of CaCancer medicine · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

H DingFaculty of Basic Medical Sciences, of Guilin Medical College, Guilin 541199, China.
X LiSchool of Pharmacy, of Guilin Medical College, Guilin 541199, China.
L ZhouSchool of Pharmacy, of Guilin Medical College, Guilin 541199, China.
Z CuiFaculty of Basic Medical Sciences, of Guilin Medical College, Guilin 541199, China.
H MengSchool of Intelligent Medicine and Biotechnology, of Guilin Medical College, Guilin 541199, China.
J WangFaculty of Basic Medical Sciences, of Guilin Medical College, Guilin 541199, China.
Affiliated Hospital of Guilin Medical College · CNGuilin Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the effect of ANP32A silencing on invasion and migration of colon cancer cells and the influence of the activity of AKT signaling pathway on this effect.

methodsColorectal cancer HCT116 and SW480 were transfected with a small interfering RNA targeting ANP32A via a lentiviral vector. At 24, 48 and 72 h after the transfection, the changes in cell proliferation and AKT activity in the cells were detected using MTT assay and Western blotting, respectively. HCT116 and SW480 cells were treated with the AKT agonist SC79 or its inhibitor MK2206 for 24, 48, 72 and 96 h, and the changes in cell migration and invasion ability were analyzed using Transwell chamber assay and cell proliferation was assessed using MTT assay. The effects of SC79 and MK2206 on migration and invasion abilities of HCT116 and SW480 cells with or without ANP32A silencing were examined using wound healing and Transwell chamber assays, and the changes in the expression of metadherin (MTDH), a factor associated with cells invasion and migration, was detected with Western blotting.

resultsLentivirus-mediated ANP32A silencing significantly down-regulated the activity of AKT and inhibited the proliferation of both HCT116 and SW480 cells (

conclusionANP32A silencing inhibits invasion and migration of colorectal cancer cells possibly by inhibiting the activation of the AKT signaling pathway.

Indexed as

Colonic NeoplasmsProto-Oncogene Proteins c-aktBlotting, WesternCell MovementCell ProliferationHumansMembrane ProteinsNuclear ProteinsRNA-Binding ProteinsANP32A protein, humanMembrane ProteinsMTDH protein, humanNuclear ProteinsProto-Oncogene Proteins c-aktRNA-Binding ProteinsAKTANP32Acolon cancerinvasionmigration

Identifiers

PMID36856210
PMCPMC9978733
OpenAlexW4322719264

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.