Evidence map›Paper›PMID 36856208›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2023

[Teriparatide regulates osteoblast differentiation in high-glucose microenvironment through the cAMP/PKA/CREB signaling pathway].

T Hou, Y Qin, Y Zhang, G Wen, M Qi, W Dong

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Exploration of the Mechanisms ofCurrent issues in molecular biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

T HouSchool of Stomatology, North China University of Science and Technology, Tangshan 063210, China.
Y QinSchool of Stomatology, North China University of Science and Technology, Tangshan 063210, China.
Y ZhangSchool of Stomatology, North China University of Science and Technology, Tangshan 063210, China.
G WenSchool of Stomatology, North China University of Science and Technology, Tangshan 063210, China.
M QiSchool of Stomatology, North China University of Science and Technology, Tangshan 063210, China.
W DongSchool of Stomatology, North China University of Science and Technology, Tangshan 063210, China.
North China University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the effect of teriparatide on the differentiation of MC3T3-E1 cells in high-glucose microenvironment and explore the possible mechanism.

methodsMC3T3-E1 cells cultured in normal glucose or high-glucose (25 mmol/L) medium were treated with 10 nmol/L teriparatide with or without co-treatment with H-89 (a PKA inhibitor). CCK-8 assay was used to detect the changes in cell proliferation, and cAMP content in the cells was determined with ELISA. Alkaline phosphatase (ALP) activity and mineralized nodules in the cells were detected using ALP kit and Alizarin red staining, respectively. The changes in cell morphology were detected by cytoskeleton staining. Real-time PCR was used to detect the mRNA expressions of PKA, CREB, RUNX2 and Osx in the treated cells.

resultsThe treatments did not result in significant changes in proliferation of MC3T3-E1 cells (

conclusionTeriparatide can promote osteoblast differentiation of MC3T3-E1 cells in high-glucose microenvironment possibly by activating the cAMP/PKA/CREB signaling pathway.

Indexed as

Core Binding Factor Alpha 1 SubunitOsteoblastsTeriparatideAnimalsCell DifferentiationCell LineGlucoseIsoquinolinesMiceRNA, MessengerSignal TransductionSulfonamidesCore Binding Factor Alpha 1 SubunitGlucoseIsoquinolinesN-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamideRNA, MessengerSulfonamidesTeriparatidecAMP/PKA/CREB signaling pathwayscell differentiationhigh-glucose microenvironmentosteoblastsosteoporosisteriparatide

Identifiers

PMID36856208
PMCPMC9978729
OpenAlexW4322720074

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.