ArticleNature communications2023
Identification of a physiologic vasculogenic fibroblast state to achieve tissue repair.
Abstract read
In one paragraph
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Reimagining VEGF Therapy: The Role of PLCγ2 in Pan-Organ Endothelial Networks.Advances in wound care · 2026Article
- Diabetic Wound Vasculopathy and Neuropathy: Spotlight on Wound Lipid Signaling.Advances in wound care · 2026Review
- Identification of Skin Multicellular Reprogramming Factors as Potential Treatment for Nonhealing Diabetic Foot Ulcers.Advances in wound care · 2026Article
- Microneedle Strategies for Diabetic Wound Management: A Comprehensive Review of Materials, Mechanisms, and Therapeutic Outcomes.Materials today. Advances · 2026Article
- Direct in vivo reprogramming to relieve tissue ischemia via induced vasculogenesis.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Review
- Cell and tissue reprogramming: Unlocking a new era in medical drug discovery.Pharmacological reviews · 2025Review
- Single-cell chromatin landscapes associated with the burnt skin healing process in rats.Scientific data · 2025Article
- Nanomanaging Chronic Wounds with Targeted Exosome Therapeutics.Pharmaceutics · 2025Review
- Immune-privileged cord blood-derived endothelial colony-forming cells: advancing immunomodulation and vascular regeneration.Angiogenesis · 2025Review
- Tissue nanotransfection-based endothelial PLCγ2-targeted epigenetic gene editing rescues perfusion and diabetic ischemic wound healing.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- miR-200c inhibition and catalase accelerate diabetic wound healing.Journal of biomedical science · 2025Article
- Antigen-presenting fibroblasts: emerging players in immune modulation and therapeutic targets.Theranostics · 2025Review
- Vasculogenic skin reprogramming requires TET-mediated gene demethylation in fibroblasts for rescuing impaired perfusion in diabetes.Nature communications · 2024Article
- Mitochondrial Bioenergetics of Functional Wound Closure is Dependent on Macrophage-Keratinocyte Exosomal Crosstalk.ACS nano · 2024Article
- Impact of Epigenetics, Diet, and Nutrition-Related Pathologies on Wound Healing.International journal of molecular sciences · 2024Review
- Subpopulations of fibroblasts derived from human iPS cells.Communications biology · 2024Article
- Fibroblast: A Novel Target for Autoimmune and Inflammatory Skin Diseases Therapeutics.Clinical reviews in allergy & immunology · 2024Review
- Tissue Nanotransfection Silicon Chip and Related Electroporation-Based Technologies for In Vivo Tissue Reprogramming.Nanomaterials (Basel, Switzerland) · 2024Review
- Acoustofluidic Engineering of Functional Vessel-on-a-Chip.ACS biomaterials science & engineering · 2023Article
- Human fetal dermal fibroblast-myeloid cell diversity is characterized by dominance of pro-healing Annexin1-FPR1 signaling.iScience · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
33 authors.
Durba Pal *Indiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Subhadip Ghatak *Indiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Kanhaiya Singh *Indiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Ahmed Safwat AbouhashemIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Manishekhar KumarIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Mohamed S El MasryIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.ORCID 0000-0002-2686-1509
Sujit K MohantyIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Ravichand PalakurtiIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Yashika RustagiIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Saba TabasumIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Dolly K KhonaIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.ORCID 0000-0001-9961-1351
Savita KhannaIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Sedat KacarIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.ORCID 0000-0002-0671-8529
Rajneesh SrivastavaIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Pramod BhasmeIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Sumit S VermaIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Edward HernandezIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Anu SharmaIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Diamond ReeseIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Priyanka VermaIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Nandini GhoshIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Mahadeo GorainIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.ORCID 0000-0003-3133-425X
Jun WanCenter for Computational Biology and Bioinformatics (CCBB), Indiana University School of Medicine, Indianapolis, IN, 46202, USA.ORCID 0000-0001-9286-6562
Sheng LiuCenter for Computational Biology and Bioinformatics (CCBB), Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Yunlong LiuCenter for Computational Biology and Bioinformatics (CCBB), Indiana University School of Medicine, Indianapolis, IN, 46202, USA.ORCID 0000-0002-2699-626X
Natalia Higuita CastroDepartment of Biomedical Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Surya C GnyawaliIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.ORCID 0000-0002-4214-8068
William LawrenceDepartment of Surgery, The Ohio State University, Columbus, OH, 43210, USA.ORCID 0000-0001-8639-3420
Jordan MooreDepartment of Surgery, The Ohio State University, Columbus, OH, 43210, USA.
Daniel Gallego PerezDepartment of Surgery, The Ohio State University, Columbus, OH, 43210, USA.ORCID 0000-0002-6263-3191
Sashwati RoyIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.
Mervin C YoderIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.ORCID 0000-0003-4354-7631
Chandan K SenIndiana Center for Regenerative Medicine & Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA. cksen@iu.edu.ORCID 0000-0003-3151-5202
Funding
Vitamin E Neuroprotection: Novel Molecular MechanismsR01NS042617 · NINDS · OHIO STATE UNIVERSITY · PI SEN, CHANDAN K · 2002 to 2021
$3.9MTissue Oxygenation and Wound HealingR01GM077185 · NIGMS · OHIO STATE UNIVERSITY · PI SEN, CHANDAN K · 2007 to 2016
$2.3MiGOT neuroprotectionR01NS085272 · NINDS · OHIO STATE UNIVERSITY · PI KHANNA, SAVITA, RINK, CAMERON LUCIAN · 2013 to 2017
$1.7MMechanisms Underlying Impaired Diabetic Wound HealingR01DK114718 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI ROY, SASHWATI · 2018 to 2021
$1.6MTissue reprogramming in diabetic wound healingR01DK128845 · NIDDK · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ROY, SASHWATI, SEN, CHANDAN K · 2021 to 2023
$1.5MMechanisms underlying impaired diabetic wound healingR01DK076566 · NIDDK · OHIO STATE UNIVERSITY · PI ROY, SASHWATI · 2008 to 2011
$1.2MNovel Regulators of Wound AngiogenesisR01GM108014 · NIGMS · OHIO STATE UNIVERSITY · PI SEN, CHANDAN K · 2015 to 2018
$1.2MDiabetic Foot Ulcer Wound Fluid BiomarkerR61DK131909 · NIDDK · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ROY, SASHWATI · 2022 to 2023
$991kMechanisms underlying impaired diabetic wound healingR56DK076566 · NIDDK · OHIO STATE UNIVERSITY · PI ROY, SASHWATI · 2016 to 2016
$347kNIDDK NIH HHS R01 DK076566NIDDK NIH HHS R01 DK114718NIDDK NIH HHS R01 DK128845NIDDK NIH HHS R56 DK076566NIDDK NIH HHS R61 DK131909NIGMS NIH HHS R01 GM077185NIGMS NIH HHS R01 GM108014NINDS NIH HHS R01 NS042617NINDS NIH HHS R01 NS085272
Abstract
Tissue injury to skin diminishes miR-200b in dermal fibroblasts. Fibroblasts are widely reported to directly reprogram into endothelial-like cells and we hypothesized that miR-200b inhibition may cause such changes. We transfected human dermal fibroblasts with anti-miR-200b oligonucleotide, then using single cell RNA sequencing, identified emergence of a vasculogenic subset with a distinct fibroblast transcriptome and demonstrated blood vessel forming function in vivo. Anti-miR-200b delivery to murine injury sites likewise enhanced tissue perfusion, wound closure, and vasculogenic fibroblast contribution to perfused vessels in a FLI1 dependent manner. Vasculogenic fibroblast subset emergence was blunted in delayed healing wounds of diabetic animals but, topical tissue nanotransfection of a single anti-miR-200b oligonucleotide was sufficient to restore FLI1 expression, vasculogenic fibroblast emergence, tissue perfusion, and wound healing. Augmenting a physiologic tissue injury adaptive response mechanism that produces a vasculogenic fibroblast state change opens new avenues for therapeutic tissue vascularization of ischemic wounds.
Indexed as
FibroblastsSkinWound HealingAnimalsAntagomirsHumansMiceMicroRNAsOligonucleotidesAntagomirsMicroRNAsMIRN200 microRNA, humanOligonucleotides
Identifiers
What OpenQuestion holds
Textmetadata
LicenceCC BY
Read underepoch 390
Registered trials
None linked
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.