Evidence map›Paper›PMID 36851445›Full record

ArticleVeterinary sciences2023

An Improved Duplex Real-Time Quantitative RT-PCR Assay with a Canine Endogenous Internal Positive Control for More Sensitive and Reliable Detection of Canine Parainfluenza Virus 5.

Gyu-Tae Jeon, Hye-Ryung Kim, Yeun-Kyung Shin, Oh-Kyu Kwon, Hae-Eun Kang, Oh-Deog Kwon, Choi-Kyu Park

Open access · goldAbstract read
In one paragraph

Article in Veterinary sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Gyu-Tae JeonAnimal Disease Intervention Center, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.ORCID 0000-0001-6912-8641
Hye-Ryung KimAnimal Disease Intervention Center, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
Yeun-Kyung ShinForeign Animal Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.
Oh-Kyu KwonForeign Animal Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.
Hae-Eun KangForeign Animal Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.ORCID 0000-0002-6013-484X
Oh-Deog KwonAnimal Disease Intervention Center, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
Choi-Kyu ParkAnimal Disease Intervention Center, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
Kyungpook National University · KRAnimal and Plant Quarantine Agency · KR

Funding

Animal and Plant Quarantine Agency Z-1543085-2022-23-03Commercialization Promotion Agency for R&D Outcomes (COMPA) grant funded by the Korean Government (Ministry of Science and ICT) R&D project No. 1711139487
6 · The paper itself

Abstract

A duplex real-time quantitative reverse transcription-polymerase chain reaction (dqRT-PCR) assay was successfully developed to simultaneously detect canine parainfluenza virus 5 (CPIV5) and a canine endogenous internal positive control (EIPC) in canine clinical samples. Two sets of primers and probes for the CPIV5 L and canine 16S rRNA genes were included in the dqRT-PCR assay to detect CPIV and monitor invalid results throughout the qRT-PCR process. The developed dqRT-PCR assay specifically detected CPIV5 but no other canine pathogens. Furthermore,

Indexed as

canine parainfluenza virus 5CIRDduplex real-time quantitative RT-PCRendogenous internal positive controlL gene

Identifiers

PMID36851445
PMCPMC9965950
OpenAlexW4319982043

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.