ArticleBMC microbiology2023
Insights into the enumeration of mixtures of probiotic bacteria by flow cytometry.
Article in BMC microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Survival of Lacticaseibacillus rhamnosus LRB in a Potential Synbiotic Oat Beverage: Insights into Cell-Matrix Interactions During In Vitro Digestion.Plant foods for human nutrition (Dordrecht, Netherlands) · 2026Article
- Polymer-Based and Biomaterial Encapsulation of Probiotics for Improved Viability.Probiotics and antimicrobial proteins · 2026Review
- Next-Generation Microencapsulation Technologies for Probiotic Protection and Precision Delivery.Microbial biotechnology · 2026Review
- Disease-modifying efficacy of alginate-microencapsulated probiotics in an animal model of ulcerative colitis.Archives of microbiology · 2026Article
- Evaluating the Quality of Selected Commercial Probiotic Products, Both Dietary Supplements and Foods for Special Medical Purposes.Foods (Basel, Switzerland) · 2026Article
- Probiotic Viability Reconsidered: Integrating VBNC Resuscitation and Culture-Independent Methods for Accurate Probiotic Enumeration.Microorganisms · 2025Review
- Metabolic characterization of alkane monooxygenases and the growth phenotypes ofJournal of bacteriology · 2025Article
- Specific cultivation-independent enumeration of viable cells in probiotic products using a combination of fluorescenceFrontiers in microbiology · 2024Article
- The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytesFrontiers in microbiomes · 2024Article
- Collaborative cytometric inter-laboratory ring test for probiotics quantification.Frontiers in microbiology · 2023Article
- Harnessing the potential of probiotics in the treatment of alcoholic liver disorders.Frontiers in pharmacology · 2023Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of flow cytometry to enumerate microorganisms is gaining traction over the traditional plate count technique on the basis of superior accuracy, precision and time-to-result. Here, we assessed the suitability of live/dead flow cytometry for the enumeration of mixed populations of probiotic bacteria (L. acidophilus, L. paracasei, L. plantarum, L. salivarius, B. lactis and B. bifidum) whilst comparing outcomes with plate counting. Using a novel gating strategy designed specifically for the enumeration of mixed populations, the application of flow cytometry resulted in the detection of higher numbers of viable bacteria with a greater level of repeatability than plate counting (RSD of 6.82 and 13.14% respectively). Across all multi-species blends tested, viable cell input was more accurately recovered by flow cytometry (101.8 ± 6.95%) than plate counts (81.37 ± 16.03%). However, when certain probiotic mixtures contained preparations with high numbers of non-viable cells in their total population, flow cytometry had the potential for overestimation of the viable population. Nevertheless, the comparative plate counts of these mixtures were low and variable, thus supporting the use of flow cytometry for the enumeration of viable bacteria in mixed populations.
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