ArticleAnalytical chemistry2023
Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies.
Article in Analytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- On-Demand Viscosity Gradients Turn Nanoliter Droplets into Programmable Sources of Enriched Picoliter Droplets.Journal of the American Chemical Society · 2026Article
- High-Throughput Screening of Isomeric Reaction Products by Droplet Microfluidics Coupled to Cyclic Ion Mobility-Mass Spectrometry.Analytical chemistry · 2026Article
- PRISM: A unified platform for phage isolation and characterization from single-droplet microenvironments.Science advances · 2026Article
- On-Demand Droplet Routing and Splitting Using Independently Addressable Interdigitated Electrodes.Micromachines · 2026Article
- Time resolved luminescence of millisecond lifetime dyes in droplet microfluidic systems.Biomicrofluidics · 2026Article
- Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Confocal Absorbance-Activated Droplet Sorting (cAADS) for Enzyme Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Microfluidic-Assisted Evolution of a Robust NADChemSusChem · 2025Article
- A Microfluidics-Based Ultrahigh-Throughput Screening Unveils Diverse Ketoreductases Relevant to Pharmaceutical Synthesis.Analytical chemistry · 2025Article
- Continuous FACS Sorting of Double Emulsion Picoreactors with a 3D-Printed Vertical Mixer.Analytical chemistry · 2025Article
- Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets.Journal of the American Chemical Society · 2025Article
- Continuous FACS sorting of double emulsion picoreactors with a 3D printed vertical mixer.bioRxiv : the preprint server for biology · 2025Article
- Hyperspectral Imaging for High Throughput Optical Spectroscopy of pL Droplets.Analytical chemistry · 2025Article
- High-Throughput Absorbance-Activated Droplet Sorting for Engineering Aldehyde Dehydrogenases.Angewandte Chemie (International ed. in English) · 2024Article
- Passive Droplet Microfluidic Platform for High-Throughput Screening of Microbial Proteolytic Activity.Analytical chemistry · 2024Article
- Droplet Microfluidics for High-Throughput Screening and Directed Evolution of Biomolecules.Micromachines · 2024Review
- Carbohydrate-active enzyme (CAZyme) discovery and engineeringRSC chemical biology · 2024Review
- Recent advances in droplet sequential monitoring methods for droplet sorting.Biomicrofluidics · 2023Review
- Mass-Activated Droplet Sorting for the Selection of Lysine-ProducingAnalytical chemistry · 2023Article
- Droplet-based μChopper device with a 3D-printed pneumatic valving layer and a simple photometer for absorbance based fructosamine quantification in human serum.The Analyst · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Droplet microfluidics is a valuable method to "beat the odds" in high throughput screening campaigns such as directed evolution, where valuable hits are infrequent and large library sizes are required. Absorbance-based sorting expands the range of enzyme families that can be subjected to droplet screening by expanding possible assays beyond fluorescence detection. However, absorbance-activated droplet sorting (AADS) is currently ∼10-fold slower than typical fluorescence-activated droplet sorting (FADS), meaning that, in comparison, a larger portion of sequence space is inaccessible due to throughput constraints. Here we improve AADS to reach kHz sorting speeds in an order of magnitude increase over previous designs, with close-to-ideal sorting accuracy. This is achieved by a combination of (i) the use of refractive index matching oil that improves signal quality by removal of side scattering (increasing the sensitivity of absorbance measurements); (ii) a sorting algorithm capable of sorting at this increased frequency with an Arduino Due; and (iii) a chip design that transmits product detection better into sorting decisions without false positives, namely a single-layered inlet to space droplets further apart and injections of "bias oil" providing a fluidic barrier preventing droplets from entering the incorrect sorting channel. The updated ultra-high-throughput absorbance-activated droplet sorter increases the effective sensitivity of absorbance measurements through better signal quality at a speed that matches the more established fluorescence-activated sorting devices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.