ArticleAnalytical and bioanalytical chemistry2023
Rapid detection of three mycotoxins in animal feed materials using competitive ELISA-based origami microfluidic paper analytical device (μPAD).
Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 30 citations in OpenAlex.
- Emerging biosensors integrated with microfluidic devices: a promising analytical tool for on-site detection of mycotoxins.NPJ science of food · 2025Review
- Comparison of protein immobilization methods with covalent bonding on paper for paper-based enzyme-linked immunosorbent assay.Analytical and bioanalytical chemistry · 2024Article
- Sustainable Sensing with Paper Microfluidics: Applications in Health, Environment, and Food Safety.Biosensors · 2024Review
- Advancing Mycotoxin Detection: Multivariate Rapid Analysis on Corn Using Surface Enhanced Raman Spectroscopy (SERS).Toxins · 2023Article
- Review
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Authors and funding
4 authors at 3 institutions in 1 country.
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Abstract
We report the development of a competitive ELISA-based origami microfluidic paper-based analytical device (μPAD) for the detection of mycotoxins in animal feed material. The μPAD was patterned using the wax printing technique with the design of a testing pad in the middle and two absorption pads at the side. Anti-mycotoxin antibodies were effectively immobilized on chitosan-glutaraldehyde-modified sample reservoirs in the μPAD. The determination of zearalenone, deoxynivalenol, and T-2 toxin in corn flour was successfully achieved by performing competitive ELISA on the μPAD in 20 min. Colorimetric results were easily distinguished by the naked eye with a detection limit of 1 µg/mL for all three mycotoxins. The μPAD integrated with competitive ELISA holds potential for practical applications in the livestock industry for rapid, sensitive, and cost-effective detection of different mycotoxins in animal feed materials.
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