Evidence map›Paper›PMID 36847541›Full record

ArticleApplied and environmental microbiology2023

Improved 2α-Hydroxylation Efficiency of Steroids by CYP154C2 Using Structure-Guided Rational Design.

Qilin Gao, Bingbing Ma, Qianwen Wang, Hao Zhang, Shinya Fushinobu, Jian Yang, Susu Lin, Keke Sun, Bing-Nan Han, Lian-Hua Xu

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.0field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Qilin Gao *College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.
Bingbing Ma *Research Center for Clinical Pharmacy, The First Affiliated Hospital & Institute of Pharmaceutical Biotechnology, School of Medicine, Zhejiang University, Hangzhou, China.
Qianwen WangOcean College, Zhejiang University, Zhoushan, China.
Hao ZhangDrug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
Shinya FushinobuDepartment of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan.
Jian YangCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.
Susu LinCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.
Keke SunCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.
Bing-Nan HanCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.
Lian-Hua XuCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.ORCID 0000-0002-9259-4429
Zhejiang Sci-Tech University · CNChinese Academy of Sciences · CNThe University of Tokyo · JPZhejiang Ocean University · CNZhejiang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cytochrome P450 enzymes are promising biocatalysts for industrial use because they catalyze site-selective C-H oxidation and have diverse catalytic reactions and a broad substrate range. In this study, the 2α-hydroxylation activity of CYP154C2 from Streptomyces avermitilis MA-4680T toward androstenedione (ASD) was identified by an

Indexed as

Cytochrome P-450 Enzyme SystemSteroidsHydroxylationOxidation-ReductionSubstrate SpecificityTestosteroneCytochrome P-450 Enzyme SystemSteroidsTestosteroneandrostenedionecrystal structureCYP154C2P450rational designtestosterone

Identifiers

PMID36847541
PMCPMC10056965
OpenAlexW4322494188

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.