ArticleFrontiers in veterinary science2023
Novel p22 and p30 dual-proteins combination based indirect ELISA for detecting antibodies against African swine fever virus.
Article in Frontiers in veterinary science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Development and Evaluation of Three Indirect ELISA Methods Based on Novel Recombinant ASFV Antigens for Serological Detection of African Swine Fever.Pathogens (Basel, Switzerland) · 2026Article
- Novel B cell epitopes mapping in pD205R protein of African swine fever virus using monoclonal antibodies.BMC veterinary research · 2026Article
- Indirect ELISA Based on ASFV Polymerase Three Subunits for Serological Monitoring of African Swine Fever Antibodies.Veterinary sciences · 2026Article
- A Novel In-Cell ELISA With Superior Sensitivity and Specificity for the Detection of African Swine Fever Virus-Specific IgM and IgG Antibodies.Transboundary and emerging diseases · 2026Article
- Accelerated aptamer selectionRSC advances · 2025Article
- Identification and Characterization of Three Novel B-Cell Epitopes in African Swine Fever Virus p22 Protein.Microorganisms · 2025Article
- Development and Standardization of Indirect ELISA for African Swine Fever Virus Using Recombinant p30 Protein Produced in Prokaryotic System.Veterinary sciences · 2025Article
- Cloning and Expression of a Truncated Form of the p72 Protein of the African Swine Fever Virus (ASFV) for Application in an Efficient Indirect ELISA System.Pathogens (Basel, Switzerland) · 2025Article
- Comparison of immunogenicity of 17 Burkholderia mallei antigens and whole cell lysate using indirect ELISA.The Journal of veterinary medical science · 2025Article
- A triple protein-based ELISA for differential detection of ASFV antibodies.Frontiers in veterinary science · 2024Article
- A Robust Quadruple Protein-Based Indirect ELISA for Detection of Antibodies to African Swine Fever Virus in Pigs.Microorganisms · 2023Article
- Review
- Establishment of a Dual-Antigen Indirect ELISA Based on p30 and pB602L to Detect Antibodies against African Swine Fever Virus.Viruses · 2023Article
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Authors and funding
15 authors.
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Abstract
Introduction: African swine fever virus (ASFV) infection is one of the most complex and fatal hemorrhagic viral diseases, causing a devastating loss to the swine industry. Since no effective vaccine is available, prevention and control of ASFV heavily depends on early diagnostic detection. Methods: In this study, a novel indirect ELISA was established for detecting antibodies against ASFV using dual-proteins, p22 and p30. Recombinants p22 and p30 were expressed and purified from Results: Through optimizing coating concentrations of p30 and p22, coating ratio (p30: p22 = 1:3), and serum dilution (as 1:600), the established ELISA performed higher specificity, sensitivity, and repeatability against ASFV-positive serum. Furthermore, 184 clinical serum samples from suspected diseased pigs were verified the established ELISA in clinical diagnosis. The results showed that compared with two commercial ELISA kits, the established ELISA possessed higher sensitivity and almost uniform coincidence rate. Conclusion: The novel indirect ELISA based on dual-proteins p30 and p22 performed a valuable role in diagnostic detection of ASFV, providing a broad insight into serological diagnostic methods of ASFV.
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