Evidence map›Paper›PMID 36846044›Full record

ArticleJournal of veterinary research2022

Evaluation of a Real-time PCR in Combination with a Cultivation Method for the Detection of

Viviana Manzulli, Valeria Rondinone, Luigina Serrecchia, Antonio Petrella, Domenico Scaltrito, Leonardo Marino, Lorenzo Pace, Maria Luigia Prencipe, Dora Cipolletta, Mauro Nitti and 3 more

Abstract read
In one paragraph

Article in Journal of veterinary research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Identification ofItalian journal of food safety · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Viviana ManzulliIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Valeria RondinoneIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Luigina SerrecchiaIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Antonio PetrellaIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Domenico ScaltritoIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Leonardo MarinoIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Lorenzo PaceIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Maria Luigia PrencipeIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Dora CipollettaIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Mauro NittiAzienda Sanitaria Locale Avellino, 83100 Avellino, Italy.
Antonio FasanellaIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Elena PoppaIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.
Domenico GalanteIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Brucellosis is a widespread zoonosis of great economic importance for livestock farming in many areas of the world. It is a highly infectious disease which is diagnosed using conventional serological and microbiological methods. The aim of this study was to assess the efficiency of a specific real-time PCR in combination with broth cultivation in detecting Material and Methods: We examined 67 organs collected from 10 cattle slaughtered following a brucellosis outbreak which occurred in February 2016 in southern Italy. The research was carried out by enrichment broth cultivations in combination with a real-time PCR every week for six weeks. Results: Conclusion: Real-time PCR has allowed results to be obtained faster than in the classical microbiological method, reducing the response times to identify positive animals by half.

Indexed as

Brucella abortusdiagnosisreal-time PCR

Identifiers

PMID36846044
PMCPMC9944991

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.