Evidence map›Paper›PMID 36845537›Full record

ArticleBio-protocol2023

Far-western Blotting Detection of the Binding of Insulin Receptor Substrate to the Insulin Receptor.

Jinghua Peng, Balamurugan Ramatchandirin, Alexia Pearah, Ling He

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Jinghua PengInstitute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Balamurugan RamatchandirinDepartment of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Alexia PearahDepartment of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Ling HeDepartment of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Johns Hopkins Medicine · USJohns Hopkins University · USShuguang Hospital · CN

Funding

Coactivator P300 promotes hepatic steatosis in obesityR01DK120309 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI HE, LING, YU, LIQING · 2019 to 2023
$2.7M
Activation of the cAMP-PKA pathway antagonizes metformin actionR01DK107641 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI HE, LING · 2016 to 2020
$1.8M
NIDDK NIH HHS R01 DK107641NIDDK NIH HHS R01 DK120309
6 · The paper itself

Abstract

Far-western blotting, derived from the western blot, has been used to detect interactions between proteins in vitro, such as receptor-ligand interactions. The insulin signaling pathway plays a critical role in the regulation of both metabolism and cell growth. The binding of the insulin receptor substrate (IRS) to the insulin receptor is essential for the propagation of downstream signaling after the activation of the insulin receptor by insulin. Here, we describe a step-by-step far-western blotting protocol for determining the binding of IRS to the insulin receptor.

Indexed as

Insulin receptorInsulin receptor substratePost-translational modificationProtein–protein bindingSignal transduction

Identifiers

PMID36845537
PMCPMC9947543
OpenAlexW4320923520

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.