Evidence map›Paper›PMID 36845093›Full record

ArticleFrontiers in immunology2023

Immunity of turbot Induced by inactivated vaccine of

Yingrui Li, Lin Su, Xiaofei Liu, Huimin Guo, Shun Zhou, Yunji Xiu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Yingrui LiInstitute of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China.
Lin SuInstitute of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China.
Xiaofei LiuInstitute of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China.
Huimin GuoInstitute of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China.
Shun ZhouInstitute of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China.
Yunji XiuInstitute of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China.
Qingdao Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: DNA methylation was one of the most important modification in epigenetics and played an important role in immune response. Since the introduction of Methods: In this study, differentially methylated regions (DMRs) were screened by Whole Genome Bisulfite Sequencing (WGBS) and significantly differentially expressed genes (DEGs) were screened by Transcriptome sequencing. Double luciferase report assay and DNA pull-down assay were further verified the DNA methylation state of the gene promoter region affected genes transcriptional activity after immunization with inactivated vaccine of Results: A total of 8149 differentially methylated regions (DMRs) were screened, in which there were many immune-related genes with altered DNA methylation status. Meanwhile, 386 significantly differentially expressed genes (DEGs) were identified, many of which were significantly enriched in Toll-like receptor signaling pathway, NOD-like receptor signaling pathway and C-type lectin receptor signaling pathway. Combined analysis of WGBS results and RNA-seq results, a total of 9 DMRs of negatively regulated genes are located in the promoter region, including 2 hypermethylated genes with lower expression and 7 hypomethylated genes with higher expression. Then, two immune-related genes C5a anaphylatoxin chemotactic receptor 1-like ( Discussion: We jointly analyzed WGBS and RNA-seq results and revealed the immune mechanism that occurred in turbot after immunized with inactivated vaccine of

Indexed as

Aeromonas salmonicidaFlatfishesAnimalsDNA MethylationEpigenesis, GeneticVaccines, InactivatedVaccines, InactivatedAeromonas salmonicidaDNA methylationinactivated vaccineScophthalmus maximustranscriptome sequencingWhole Genome Bisulfite Sequencing

Identifiers

PMID36845093
PMCPMC9945314
OpenAlexW4319601760

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.