Evidence map›Paper›PMID 36845033›Full record

ArticleiScience2023

Demultiplexing Ig repertoires by parallel mRNA/DNA sequencing shows major differential alterations in severe COVID-19.

Virginie Pascal, Marine Dupont, Paco de Rouault, David Rizzo, Delphine Rossille, Robin Jeannet, Thomas Daix, Bruno François, Steve Genebrier, Marie Cornic and 10 more

Registry-linked trialOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04373200 (Human Ab Response & immunoMONItoring of COVID-19 Patients), which is not on this map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04373200 nacompletednot on this map

Human Ab Response & immunoMONItoring of COVID-19 Patients

TypeinterventionalSponsorRennes University HospitalRan2020 to 2021Enrolled71ConditionsSARS-CoV-2 Coronavirus, Acute Respiratory Distress SyndromeArmsBlood samples collection, Saliva collection
3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 6 institutions in 1 country.

Virginie PascalCNRS UMR-7276, INSERM U1262, Team 3 BioPIC of CRIBL, University of Limoges, and Immunology Laboratory of Dupuytren Hospital University Center (CHU) of Limoges, Limoges, France.
Marine DupontCNRS UMR-7276, INSERM U1262, Team 1 2MB2C of CRIBL, University of Limoges, and Hematology Laboratory of Dupuytren Hospital University Center (CHU) of Limoges, Limoges, France.
Paco de RouaultDepartment of BioInformatics of Dupuytren Hospital University Center (CHU) of Limoges, Limoges, France.
David RizzoCNRS UMR-7276, INSERM U1262, Team 1 2MB2C of CRIBL, University of Limoges, and Hematology Laboratory of Dupuytren Hospital University Center (CHU) of Limoges, Limoges, France.
Delphine RossilleInstitut national de la santé et de la recherche médicale, Unité Mixte de Recherche U1236, LabEx IGO, Université Rennes 1, Etablissement Français du Sang Bretagne, 35000 Rennes, France.
Robin JeannetCNRS UMR-7276, INSERM U1262, Team 1 2MB2C of CRIBL, University of Limoges, and Hematology Laboratory of Dupuytren Hospital University Center (CHU) of Limoges, Limoges, France.
Thomas DaixInserm CIC 1435, UMR 1092, Faculty of Medicine, University of Limoges, and Réanimation Polyvalente, CHU Dupuytren, 2, 87042 Limoges, France.
Bruno FrançoisInserm CIC 1435, UMR 1092, Faculty of Medicine, University of Limoges, and Réanimation Polyvalente, CHU Dupuytren, 2, 87042 Limoges, France.
Steve GenebrierInstitut national de la santé et de la recherche médicale, Unité Mixte de Recherche U1236, LabEx IGO, Université Rennes 1, Etablissement Français du Sang Bretagne, 35000 Rennes, France.
Marie CornicCentre Hospitalier Universitaire de Rennes, SITI, Pôle Biologie, 35033 Rennes, France.
Guillaume MonneretCellular Immunology Laboratory, Hospices Civils de Lyon, Hôpital Edouard Herriot, Lyon, France.
Fabienne VenetCellular Immunology Laboratory, Hospices Civils de Lyon, Hôpital Edouard Herriot, Lyon, France.
Juliette FerrantInstitut national de la santé et de la recherche médicale, Unité Mixte de Recherche U1236, LabEx IGO, Université Rennes 1, Etablissement Français du Sang Bretagne, 35000 Rennes, France.
Mikael RousselInstitut national de la santé et de la recherche médicale, Unité Mixte de Recherche U1236, LabEx IGO, Université Rennes 1, Etablissement Français du Sang Bretagne, 35000 Rennes, France.
Florian ReizineInstitut national de la santé et de la recherche médicale, Unité Mixte de Recherche U1236, LabEx IGO, Université Rennes 1, Etablissement Français du Sang Bretagne, 35000 Rennes, France.
Mathieu Le SouhaitierInstitut national de la santé et de la recherche médicale, Unité Mixte de Recherche U1236, LabEx IGO, Université Rennes 1, Etablissement Français du Sang Bretagne, 35000 Rennes, France.
Jean-Marc TadiéInstitut national de la santé et de la recherche médicale, Unité Mixte de Recherche U1236, LabEx IGO, Université Rennes 1, Etablissement Français du Sang Bretagne, 35000 Rennes, France.
Karin TarteInstitut national de la santé et de la recherche médicale, Unité Mixte de Recherche U1236, LabEx IGO, Université Rennes 1, Etablissement Français du Sang Bretagne, 35000 Rennes, France.
Jean FeuillardCNRS UMR-7276, INSERM U1262, Team 1 2MB2C of CRIBL, University of Limoges, and Hematology Laboratory of Dupuytren Hospital University Center (CHU) of Limoges, Limoges, France.
Michel CognéInstitut national de la santé et de la recherche médicale, Unité Mixte de Recherche U1236, LabEx IGO, Université Rennes 1, Etablissement Français du Sang Bretagne, 35000 Rennes, France.
Établissement Français du Sang · FRCentre National de la Recherche Scientifique · FRHospices Civils de Lyon · FRInserm · FRCentre Hospitalier Universitaire de Rennes · FRHôpital Dupuytren · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To understand the fine differential elements that can lead to or prevent acute respiratory distress syndrome (ARDS) in COVID-19 patients, it is crucial to investigate the immune response architecture. We herein dissected the multiple layers of B cell responses by flow cytometry and Ig repertoire analysis from acute phase to recovery. Flow cytometry with FlowSOM analysis showed major changes associated with COVID-19 inflammation such as an increase of double-negative B-cells and ongoing plasma cell differentiation. This paralleled COVID-19-driven expansion of two disconnected B-cell repertoires. Demultiplexing successive DNA and RNA Ig repertoire patterns characterized an early expansion of IgG1 clonotypes with atypically long and uncharged CDR3, the abundance of this inflammatory repertoire being correlated with ARDS and likely pejorative. A superimposed convergent response included convergent anti-SARS-CoV-2 clonotypes. It featured progressively increasing somatic hypermutation together with normal-length or short CDR3 and it persisted until a quiescent memory B-cell stage after recovery.

Indexed as

ImmunologyRespiratory medicine

Identifiers

PMID36845033
PMCPMC9942447
OpenAlexW4321503003

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.