ArticleFrontiers in cellular and infection microbiology2023
Detection of immunoreactive proteins of
Article in Frontiers in cellular and infection microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Mass Spectrometry-Based Analysis of Surface Proteins inJournal of proteome research · 2025Article
- Nanotechnology improves the detection of bacteria: Recent advances and future perspectives.Heliyon · 2024Review
- A Rapid and Inexpensive PCR Test for Mastitis Diagnosis Based on NGS Data.Pathogens (Basel, Switzerland) · 2024Article
- Molecular and phenotypic identification of bacterial species isolated from cows with mastitis from three regions of Poland.BMC veterinary research · 2024Article
- Development of a prototypic, field-usable diagnostic tool for the detection of gram-positive cocci-induced mastitis in cattle.BMC veterinary research · 2024Article
- The Two-Track Investigation of Fibronectin Binding Protein A ofInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Mastitis is a widespread mammary gland disease of dairy cows that causes severe economic losses to dairy farms. Mastitis can be caused by bacteria, fungi, and algae. The most common species isolated from infected milk are, among others, Methods: The study group included 22 milk samples and 13 serum samples obtained from cows with diagnosed mastitis, whereas the control group constituted 12 milk samples and 12 serum samples isolated from healthy animals. Detection of immunoreactive proteins was done by immunoblotting, while amino acid sequences from investigated proteins were determined by MALDI-TOF. Then, bioinformatic analyses were performed on detected species specific proteins in order to investigate their immunoreactivity. Results: As a result, we identified 13 proteins: 3 (molybdenum cofactor biosynthesis protein B, aldehyde reductase YahK, outer membrane protein A) for Discussion: Due to the confirmed immunoreactivity, specificity and localization in the bacterial cell, these proteins can be considered considered potential targets in innovative rapid immunodiagnostic assays for bovine mastitis, however due to the limited number of examined samples, further examination is needed.
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