Evidence map›Paper›PMID 36844407›Full record

ArticleFrontiers in cellular and infection microbiology2023

Genetic and phenotypic assessment of the antimicrobial activity of three potential probiotic lactobacilli against human enteropathogenic bacteria.

Despoina Eugenia Kiousi, Christos Efstathiou, Vasilis Tzampazlis, Stavros Plessas, Maria Panopoulou, Maria Koffa, Alex Galanis

Open access · goldAbstract read
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
6.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 31 citations in OpenAlex.

  1. ProbioticCells · 2026
    Article
  2. Human colostrum as a probiotic reservoir: a genomic insights into potential probiotic traits.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
  3. Article
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  5. Screening and Characterization ofFoods (Basel, Switzerland) · 2026
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  6. Article
  7. Article
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  9. Multifunctional antimicrobial effects ofFrontiers in immunology · 2026
    Article
  10. Novel anti-virulence strategy againstFrontiers in microbiology · 2026
    Article
  11. Article
  12. Microorganisms · 2025
    Article
  13. Article
  14. Review
  15. Article
  16. Bioconversion of FePharmaceuticals (Basel, Switzerland) · 2025
    Article
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Despoina Eugenia KiousiDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece.
Christos EfstathiouDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece.
Vasilis TzampazlisDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece.
Stavros PlessasDepartment of Agricultural Development, Democritus University of Thrace, Orestiada, Greece.
Maria PanopoulouDepartment of Medicine, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece.
Maria KoffaDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece.
Alex GalanisDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece.
Democritus University of Thrace · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Lactobacilli are avid producers of antimicrobial compounds responsible for their adaptation and survival in microbe-rich matrices. The bactericidal or bacteriostatic ability of lactic acid bacteria (LAB) can be exploited for the identification of novel antimicrobial compounds to be incorporated in functional foodstuffs or pharmaceutical supplements. In this study, the antimicrobial and antibiofilm properties of Methods: The ability of viable cells to inhibit pathogen colonization on HT-29 cell monolayers, as well as their co-aggregation capacity, were examined utilizing the competitive exclusion assay. The antimicrobial activity of cell-free culture supernatants (CFCS) was determined against planktonic cells and biofilms, using microbiological assays, confocal microscopy, and gene expression analysis of biofilm formation-related genes. Furthermore, Results: The three lactobacilli were able to limit the viability of planktonic cells of Discussion: The efficiency of the potentially probiotic bacteria to elicit antimicrobial effects presented a strain- and pathogen-specific pattern. Future studies, utilizing multi-omic approaches, will focus on the structural and functional characterization of molecules involved in the recorded phenotypes.

Indexed as

Anti-Infective AgentsBacteriocinsProbioticsEscherichia coliHumansLactobacillaceaeLactobacillusSalmonella enteritidisStaphylococcus aureusAnti-Infective AgentsBacteriocinsantimicrobialbiofilmscompetitive exclusionconfocal microscopyenteropathogensin silico analysislactobacilliprobiotics

Identifiers

PMID36844407
PMCPMC9944596
OpenAlexW4319602549

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.