ArticleInternational journal of bioprinting2023
Effect of sterilization processes on alginate/gelatin inks for three-dimensional printing.
Article in International journal of bioprinting, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Photoprotection of a novel resveratrol analogue: insights from 2D, 3D, and endothelialized bioprinted human skin models.Frontiers in pharmacology · 2026Article
- Evaluation of Medical-Grade Polycaprolactone for 3D Printing: Mechanical, Chemical, and Biodegradation Characteristics.Polymers · 2025Article
- Optimization of Ink Composition and 3D Printing Process to Develop Soy Protein-Based Scaffolds.Gels (Basel, Switzerland) · 2024Article
- Intranasal Ion-Triggered In Situ Delivery System of Virus-like Particles: Development Using the Quality by Design Approach.Polymers · 2024Article
- Alginate-Gelatin Hydrogel Scaffolds; An Optimization of Post-Printing Treatment for Enhanced Degradation and Swelling Behavior.Gels (Basel, Switzerland) · 2023Article
- Valorization of agar production residue as a filler in soy protein hydrogels for 3D printing.International journal of bioprinting · 2023Article
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Authors and funding
5 authors.
Funding
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Abstract
309Sterilization is a crucial step in the process of developing bioinks for tissue engineering applications. In this work, alginate/gelatin inks were subjected to three sterilization methods: ultraviolet (UV) radiation, filtration (FILT), and autoclaving (AUTO). In addition, to simulate the sterilization effect in a real environment, inks were formulated in two different media, specifically, Dulbecco's Modified Eagle's Medium (DMEM) and phosphate-buffered saline (PBS). First, rheological tests were performed to evaluate the flow properties of the inks, and we observed that UV samples showed shear thinning behavior, which was favorable for three-dimensional (3D) printing. Furthermore, the 3D-printed constructs developed with UV inks showed better shape and size fidelity than those obtained with FILT and AUTO. In order to relate this behavior to the material structure, Fourier transform infrared (FTIR) analysis was carried out and the predominant conformation in protein was determined by deconvolution of the amide I band, which confirmed that the prevalence of a-helix structure was greater for UV samples. This work highlights the relevance of sterilization processes, which are essential for biomedical applications, in the research field of bioinks.
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