ArticleRegenerative therapy2023
Effect of disinfectants and manual wiping for processing the cell product changeover in a biosafety cabinet.
Article in Regenerative therapy, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Biosafety-cabinet wipe-cleaning assessment system: Integration of visual, thermal, and pressure sensing.Regenerative therapy · 2026Article
- Environmental monitoring practices relevant to regenerative medicine manufacturing: Insights from a cross-sector survey of regulated facilities in Japan.Regenerative therapy · 2026Article
- Ambient intelligence-based framework for quantitative evaluation of cell culture operations to support standardization and training.Regenerative therapy · 2026Article
- Microbial contamination risks and clinical safety in platelet-rich plasma therapy and evaluation of rapid microbial detection methods.Regenerative therapy · 2026Article
- Managing and monitoring indoor air quality and surface decontamination in healthcare environments.Arhiv za higijenu rada i toksikologiju · 2025Review
- Microbiome-based profiles of airborne bacteria to support microbial risk assessment in cleanroom environments.Regenerative therapy · 2025Article
- Optimized personnel flow with minimal contamination: development and validation of an air-barrier cleanroom for cell products processing.Regenerative therapy · 2025Article
- Applicability of Controllable Normal Force Platform for Study of Bacteria Removal During Dry Cleaning in Dry Food Manufacturing Environments.Foods (Basel, Switzerland) · 2025Article
- Cleaning methods for biosafety cabinet to eliminate residual mycoplasmas, viruses, and endotoxins after changeover.Regenerative therapy · 2025Article
- Changeover method for biosafety cabinets using ozone gas.PloS one · 2025Article
- Decreasing electricity costs of clean room for cell products during non-operation.Regenerative therapy · 2024Article
- Evaluation of cleaning methods for change-over after the processing of cell products to avoid cross-contamination risk.Regenerative therapy · 2024Article
- Operator-derived particles and falling bacteria in biosafety cabinets.Regenerative therapy · 2024Article
- Molecular, Cellular, and Technical Aspects of Breast Cancer Cell Lines as a Foundational Tool in Cancer Research.Life (Basel, Switzerland) · 2023Review
- Review
- Volatile organic compounds and ionic substances contamination in cell processing facilities during rest period; a preliminary assessment of exposure to cell processing operators.Regenerative therapy · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: The process of cell product changeover poses a high risk of cross-contamination. Hence, it is essential to minimize cross-contamination while processing cell products. Following its use, the surface of a biosafety cabinet is commonly disinfected by ethanol spray and manual wiping methods. However, the effectiveness of this protocol and the optimal disinfectant have not yet been evaluated. Here, we assessed the effect of various disinfectants and manual wiping methods on bacterial removal during cell processing. Methods: The hard surface carrier test was performed to evaluate the disinfectant efficacy of benzalkonium chloride with a corrosion inhibitor (BKC + I), ethanol (ETH), peracetic acid (PAA), and wiping against Results: In total, 2.02 ± 0.21-Log and 3.00 ± 0.46-Log reductions from 6-Log CFU of Conclusions: DW and BKC + I are effective for inducing a 3-Log reduction in bacterial abundance. Moreover, the combination of optimal wet conditions and disinfectants is essential for effective wiping in specific environments containing high-protein human sera and tissues. Given that some raw materials processed in cell products contain high protein levels, our findings suggest that a complete changeover of biosafety cabinets is necessary in terms of both cleaning and disinfection.
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