Evidence map›Paper›PMID 36843032›Full record

ArticleBiological research2023

YULINK regulates vascular formation in zebrafish and HUVECs.

Hsin-Hung Lin, Ming-Wei Kuo, Tan-Chi Fan, Alice L Yu, John Yu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biological research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Hsin-Hung Lin *Chemical Biology and Molecular Biophysics Program, International Graduate Program, Academia Sinica, Taipei, Taiwan.
Ming-Wei Kuo *Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, 333, Taoyuan, Taiwan.
Tan-Chi FanInstitute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, 333, Taoyuan, Taiwan.
Alice L YuInstitute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, 333, Taoyuan, Taiwan.
John YuInstitute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, 333, Taoyuan, Taiwan. johnyu@gate.sinica.edu.tw.ORCID http://orcid.org/0000-0002-3237-4272
Chang Gung Memorial Hospital · TW

Funding

Chang Gung Medical Foundation CMRPG3F0972Chang Gung Medical Foundation CMRPG3F0973Chang Gung Medical Foundation OMRPG3C0047Chang Gung Medical Foundation OMRPG3C0048Ministry of Science and Technology, Taiwan MOST 111-2321-B-182-002
6 · The paper itself

Abstract

backgroundThe distinct arterial and venous cell fates are dictated by a combination of various genetic factors which form diverse types of blood vessels such as arteries, veins, and capillaries. We report here that YULINK protein is involved in vasculogenesis, especially venous formation.

methodsIn this manuscript, we employed gene knockdown, yeast two-hybrid, FLIM-FRET, immunoprecipitation, and various imaging technologies to investigate the role of YULINK gene in zebrafish and human umbilical vein endothelial cells (HUVECs).

resultsKnockdown of YULINK during the arterial-venous developmental stage of zebrafish embryos led to the defective venous formation and abnormal vascular plexus formation. Knockdown of YULINK in HUVECs impaired their ability to undergo cell migration and differentiation into a capillary-like tube formation. In addition, the phosphorylated EPHB4 was decreased in YULINK knockdown HUVECs. Yeast two-hybrid, FLIM-FRET, immunoprecipitation, as well as imaging technologies showed that YULINK colocalized with endosome related proteins (EPS15, RAB33B or TICAM2) and markers (Clathrin and RHOB). VEGF-induced VEGFR2 internalization was also compromised in YULINK knockdown HUVECs, demonstrating to the involvement of YULINK.

conclusionThis study suggests that YULINK regulates vasculogenesis, possibly through endocytosis in zebrafish and HUVECs.

Indexed as

Saccharomyces cerevisiaeZebrafishAnimalsCell DifferentiationCell MovementHumansHuman Umbilical Vein Endothelial CellsNeovascularization, PhysiologicClathrinEndocytosisEPS15RAB33BRHOBTICAM2VasculogenesisVEGFVEGFR2YULINK

Identifiers

PMID36843032
PMCPMC9969694
OpenAlexW4322201560

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.