ArticleMethods in molecular biology (Clifton, N.J.)2023
Visualization of Peptidoglycan Structures of Escherichia coli by Quick-Freeze Deep-Etch Electron Microscopy.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Engineering of Surface Display Systems inBiotech (Basel (Switzerland)) · 2026Article
- Visualization of peptidoglycan layer isolated from gliding diderm bacteria,Biophysics and physicobiology · 2025Article
- Visualization of Bacillus subtilis spore structure and germination using quick-freeze deep-etch electron microscopy.Microscopy (Oxford, England) · 2024Article
- Article
- Budding and explosive membrane vesicle production by hypervesiculatingFrontiers in microbiology · 2024Article
- Construction and characterization of a hypervesiculation strain of Escherichia coli Nissle 1917.PloS one · 2024Article
- Relationship between the Rod complex and peptidoglycan structure in Escherichia coli.MicrobiologyOpen · 2023Article
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Authors and funding
2 authors.
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Abstract
Peptidoglycan (PG) is an essential component of the bacterial cell wall that protects the cell from turgor pressure and maintains its shape. In diderm (gram-negative) bacteria, such as Escherichia coli, the PG layer is flexible with a thickness of a 2-6 nm, and its visualization is difficult due to the presence of the outer membrane. The quick-freeze deep-etch replica method has been widely used for the visualization of flexible structures in cell interior, such as cell organelles and membrane components. In this technique, a platinum replica on the surface of a specimen fixed by freezing is observed using a transmission electron microscope. In this chapter, we describe the application of this method for visualizing the E. coli PG layer. We expect that these methods will be useful for the visualization of the PG layer in diverse bacterial species.
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