ArticleJournal of applied microbiology2023
Expression of the 2Duf protein in wild-type Bacillus subtilis spores stabilizes inner membrane proteins and increases spore resistance to wet heat and hydrogen peroxide.
Article in Journal of applied microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Effects of levels of purity of spores of twoApplied and environmental microbiology · 2026Article
- Two new factors modulatingmSphere · 2026Article
- Role of Stress Response Genes in Resistance and Germination ofMicroorganisms · 2026Article
- The Dual Role of RsiP in Regulating Virulence and Host Adaptation inPathogens (Basel, Switzerland) · 2026Article
- Effects of sporulation times in liquid or on plates onJournal of bacteriology · 2025Article
- Resistance and germination of spores ofJournal of bacteriology · 2025Article
- Pathways for accelerated bacterial spore killing with ohmic heating.NPJ science of food · 2025Article
- Germination and Heat Resistance ofFoods (Basel, Switzerland) · 2025Article
- Article
- Synergistic effect of high-pressure thermal sterilization and muramidase on Bacillus subtilis spores: alterations in intrasporal components, inner membrane permeability, and structural integrity.Archives of microbiology · 2025Article
- Germination ofJournal of bacteriology · 2025Article
- Recent progress in proteins regulating the germination ofJournal of bacteriology · 2025Review
- Genomic identification of germinant receptors and phenotypic response to germinants inCurrent research in microbial sciences · 2025Article
- The megaplasmid pCER270 ofApplied and environmental microbiology · 2024Article
- A coating of lipoproteins provides a stabilizing environment on the inner membrane ofJournal of bacteriology · 2023Article
- Beyond analytic solution: Analysis of FRAP experiments by spatial simulation of the forward problem.Biophysical journal · 2023Article
- New Thoughts on an Old Topic: Secrets of Bacterial Spore Resistance Slowly Being Revealed.Microbiology and molecular biology reviews : MMBR · 2023Review
- Identification and characterization of new proteins crucial for bacterial spore resistance and germination.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
aimsThis work aimed to characterize spore inner membrane (IM) properties and the mechanism of spore killing by wet heat and H2O2 with spores overexpressing the 2Duf protein, which is naturally encoded from a transposon found only in some Bacillus strains with much higher spore resistance than wild-type spores. METHODS AND
resultsKilling of Bacillus subtilis spores by wet heat or hydrogen peroxide (H2O2) was slower when 2Duf was present, and Ca-dipicolinic acid release was slower than killing. Viabilities on rich plates of wet heat- or H2O2 -treated spores +/- 2Duf were lower when NaCl was added, but higher with glucose. Addition of glucose but not Casamino acids addition increased treated spores' viability on minimal medium plates. Spores with 2Duf required higher heat activation for germination, and their germination was more wet-heat resistant than that of wild-type spores, processes that involve IM proteins. IM permeability and lipid mobility were lower in spores with 2Duf, although IM phospholipid composition was similar in spores +/- 2Duf.
conclusionsThese results and previous work suggests that wet heat and H2O2 kill spores by damaging an IM enzyme or enzymes involved in oxidative phosphorylation.
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