Evidence map›Paper›PMID 36840712›Full record

ArticleNucleic acids research2023

Red light-emitting short Mango-based system enables tracking a mycobacterial small noncoding RNA in infected macrophages.

Oksana S Bychenko, Alexei A Khrulev, Julia I Svetlova, Vladimir B Tsvetkov, Polina N Kamzeeva, Yulia V Skvortsova, Boris S Tupertsev, Igor A Ivanov, Leonid V Aseev, Yuriy M Khodarovich and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Frontiers in cellular and infection microbiology · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 7 institutions in 1 country.

Oksana S BychenkoShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Alexei A KhrulevShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Julia I SvetlovaFederal Research and Clinical Center of Physical-Chemical Medicine, Moscow 119435, Russia.
Vladimir B TsvetkovFederal Research and Clinical Center of Physical-Chemical Medicine, Moscow 119435, Russia.ORCID 0000-0003-0494-3332
Polina N KamzeevaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Yulia V SkvortsovaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Boris S TupertsevSkolkovo Institute of Science and Technology, Moscow 121205, Russia.
Igor A IvanovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Leonid V AseevShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Yuriy M KhodarovichShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Evgeny S BelyaevFrumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow 119071, Russia.
Liubov I KozlovskayaFSBSI Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products, Russian Academy of Sciences, Moscow 108819, Russia.
Timofei S ZatsepinLomonosov Moscow State University, Department of Chemistry, Moscow 119992, Russia.
Tatyana L AzhikinaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
Anna M VarizhukFederal Research and Clinical Center of Physical-Chemical Medicine, Moscow 119435, Russia.
Andrey V AralovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.ORCID 0000-0003-3268-2352
Institute of Bioorganic Chemistry · RUFederal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin · RUFrumkin Institute of Physical Chemistry and Electrochemistry · RULomonosov Moscow State University · RURussian Academy of Sciences · RUSechenov University · RUSkolkovo Institute of Science and Technology · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Progress in RNA metabolism and function studies relies largely on molecular imaging systems, including those comprising a fluorogenic dye and an aptamer-based fluorescence-activating tag. G4 aptamers of the Mango family, typically combined with a duplex/hairpin scaffold, activate the fluorescence of a green light-emitting dye TO1-biotin and hold great promise for intracellular RNA tracking. Here, we report a new Mango-based imaging platform. Its key advantages are the tunability of spectral properties and applicability for visualization of small RNA molecules that require minimal tag size. The former advantage is due to an expanded (green-to-red-emitting) palette of TO1-inspired fluorogenic dyes, and the truncated duplex scaffold ensures the latter. To illustrate the applicability of the improved platform, we tagged Mycobacterium tuberculosis sncRNA with the shortened aptamer-scaffold tag. Then, we visualized it in bacteria and bacteria-infected macrophages using the new red light-emitting Mango-activated dye.

Indexed as

Fluorescent DyesMacrophagesMangiferaRNA, Small UntranslatedAptamers, NucleotideFluorescenceRNAAptamers, NucleotideFluorescent DyesRNARNA, Small Untranslated

Identifiers

PMID36840712
PMCPMC10085697
OpenAlexW4321995585

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.