Evidence map›Paper›PMID 36838504›Full record

ReviewMicroorganisms2023

Protoplast Preparation for Algal Single-Cell Omics Sequencing.

Junran Ye, Cuiqiyun Yang, Luojia Xia, Yinjie Zhu, Li Liu, Huansheng Cao, Yi Tao

Open access · goldAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Microorganisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Junran YeDivision of Natural and Applied Sciences, Duke Kunshan University, 8 Duke Ave, Kunshan 215316, China.ORCID 0000-0001-5199-7638
Cuiqiyun YangDivision of Natural and Applied Sciences, Duke Kunshan University, 8 Duke Ave, Kunshan 215316, China.
Luojia XiaDivision of Natural and Applied Sciences, Duke Kunshan University, 8 Duke Ave, Kunshan 215316, China.
Yinjie ZhuGroundwater Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.ORCID 0000-0001-7623-5576
Li LiuDivision of Natural and Applied Sciences, Duke Kunshan University, 8 Duke Ave, Kunshan 215316, China.
Huansheng CaoDivision of Natural and Applied Sciences, Duke Kunshan University, 8 Duke Ave, Kunshan 215316, China.
Yi TaoGroundwater Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.ORCID 0000-0001-7692-2122
Duke Kunshan University · CNUniversity Town of Shenzhen · CN

Funding

National Natural Science Foundation of China 32171565National Natural Science Foundation of China 52070117
6 · The paper itself

Abstract

Single-cell sequencing (SCS) is an evolutionary technique for conducting life science research, providing the highest genome-sale throughput and single-cell resolution and unprecedented capabilities in addressing mechanistic and operational questions. Unfortunately, the current SCS pipeline cannot be directly applied to algal research as algal cells have cell walls, which makes RNA extraction hard for the current SCS platforms. Fortunately, effective methods are available for producing algal protoplasts (cells without cell walls), which can be directly fed into current SCS pipelines. In this review, we first summarize the cell wall structure and chemical composition of algal cell walls, particularly in Chlorophyta, then summarize the advances made in preparing algal protoplasts using physical, chemical, and biological methods, followed by specific cases of algal protoplast production in some commonly used eukaryotic algae. This review provides a timely primer to those interested in applying SCS in eukaryotic algal research.

Indexed as

algal protoplastbeadcellulosecell wallChlorella vulgarisenzymatic lysissequencingsingle-cell

Identifiers

PMID36838504
PMCPMC9962006
OpenAlexW4321459863

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.