ArticleMetabolites2023
Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA.
Article in Metabolites, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 7 citations in OpenAlex.
- Antiepileptic and antiepileptogenic effects ofIBRO neuroscience reports · 2026Article
- Lactic Acid Bacteria-Derived γ-Aminobutyric Acid: From Targeted Screening and Biosynthesis to Functional Food Applications and Health Benefits.Foods (Basel, Switzerland) · 2026Review
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- Bridging PCR-Based Genome-Walking Protocol.Bio-protocol · 2025Article
- Hybrid substrate-based pH autobuffering GABA fermentation by Levilactobacillus brevis CD0817.Bioprocess and biosystems engineering · 2024Article
- Physiological responses and transcriptome analysis ofFrontiers in plant science · 2024Article
- Sodium-Ion-Free Fermentative Production of GABA withMetabolites · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gamma-aminobutyric acid (GABA) is a functional metabolite in various organisms. Herein, a sensitivity intensified ninhydrin-based chromogenic system (SINICS), achieved by ethanol and ethyl acetate, is described for the reliable relative quantitation of GABA. A 2.9 mL SINICS kit comprises 1% ninhydrin, 40% ethanol, 25% ethyl acetate, and 35 μL 0.2 M sodium acetate buffer (pH 5.0). In practice, following the addition of a 0.1 mL sample to the kit, the chromogenic reaction is completed by heating at 70 °C for 30 min. The kit increased the color development sensitivity of L-glutamic acid and GABA, with the detection limits being reduced from 20 mM and 200 mM to 5 mM and 20 mM, respectively. The chromophore was stable for at least 2 h at room temperature, which was sufficient for a routine colorimetric analysis. The absorbance at 570 nm with the deduction of background directly represents the content of amino acid. For a proof-of-concept, the SINICS was adopted to optimize the GABA fermentation process of
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