Evidence map›Paper›PMID 36836327›Full record

ArticleJournal of fungi (Basel, Switzerland)2023

Evaluation of Host Constitutive and Ex Vivo Coccidioidal Antigen-Stimulated Immune Response in Dogs with Naturally Acquired Coccidioidomycosis.

Jared A Jaffey, Lisa F Shubitz, Michael D L Johnson, Charlotte A Bolch, Anderson da Cunha, Ashlesh K Murthy, Brina S Lopez, Ross Monasky, Imani Carswell, Justine Spiker and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Jared A JaffeyDepartment of Specialty Medicine, College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
Lisa F ShubitzValley Fever Center for Excellence, College of Medicine-Tucson, University of Arizona, Tucson, AZ 85724, USA.
Michael D L JohnsonDepartment of Immunobiology, Valley Fever Center for Excellence, BIO5 Institute, Asthma and Airway Disease Research Center, University of Arizona, College of Medicine-Tucson, Tucson, AZ 85724, USA.
Charlotte A BolchOffice of Research and Sponsored Programs, College of Graduate Studies, Midwestern University, Glendale, AZ 85308, USA.
Anderson da CunhaDepartment of Specialty Medicine, College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
Ashlesh K MurthyDepartment of Pathology, College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
Brina S LopezDepartment of Pathology, College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
Ross MonaskyDepartment of Pathology, College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
Imani CarswellDepartment of Specialty Medicine, College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
Justine SpikerDepartment of Specialty Medicine, College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
Miranda J NeubertDepartment of Immunobiology, College of Medicine-Tucson, Tucson, AZ 85724, USA.
Sanjay V MenghaniDepartment of Immunobiology, Medical Scientist Training Program, College of Medicine-Tucson, Tucson, AZ 85724, USA.ORCID 0000-0003-2056-1879
Midwestern University · USUniversity of Arizona · USTucson Medical Center · US

Funding

An Avirulent Arthroconidial Vaccine Candidate to Prevent Human CoccidioidomycosisR01AI132140 · NIAID · UNIVERSITY OF ARIZONA · PI GALGIANI, JOHN N · 2017 to 2020
$4.8M
American Kennel Club Canine Health Foundation 2408NIAID NIH HHS R01 AI132140NIH HHS RO1-AI-132140
6 · The paper itself

Abstract

The early innate immune response to coccidioidomycosis has proven to be pivotal in directing the adaptive immune response and disease outcome in mice and humans but is unexplored in dogs. The objectives of this study were to evaluate the innate immune profile of dogs with coccidioidomycosis and determine if differences exist based on the extent of infection (i.e., pulmonary or disseminated). A total of 28 dogs with coccidioidomycosis (pulmonary, n = 16; disseminated, n = 12) and 10 seronegative healthy controls were enrolled. Immunologic testing was performed immediately, without ex vivo incubation (i.e., constitutive), and after coccidioidal antigen stimulation of whole blood cultures. Whole blood cultures were incubated with a phosphate-buffered solution (PBS) (negative control) or a coccidioidal antigen (rCTS1 (105-310); 10 µg/mL) for 24 h. A validated canine-specific multiplex bead-based assay was used to measure 12 cytokines in plasma and cell culture supernatant. Serum C-reactive protein (CRP) was measured with an ELISA assay. Leukocyte expression of toll-like receptors (TLRs)2 and TLR4 was measured using flow cytometry. Dogs with coccidioidomycosis had higher constitutive plasma keratinocyte chemotactic (KC)-like concentrations (

Indexed as

Coccidioides spp.cytokinedisseminatedflow cytometryinflammationpulmonaryTLR2TLR4toll-like receptor

Identifiers

PMID36836327
PMCPMC9959558
OpenAlexW4319296722

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.