Evidence map›Paper›PMID 36835581›Full record

ArticleInternational journal of molecular sciences2023

OMICS Analyses Unraveling Related Gene and Protein-Driven Molecular Mechanisms Underlying PACAP 38-Induced Neurite Outgrowth in PC12 Cells.

Junko Shibato, Fumiko Takenoya, Michio Yamashita, Ravi Gupta, Cheol Woo Min, Sun Tae Kim, Ai Kimura, Ichiro Takasaki, Motohide Hori, Seiji Shioda and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 2 countries.

Junko ShibatoDepartment of Functional Morphology, Shonan University of Medical Sciences, 16-48 Kamishinano, Totsuka-ku, Yokohama 244-0806, Japan.
Fumiko TakenoyaDepartment of Sport Sciences, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Michio YamashitaDepartment of Sport Sciences, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.ORCID 0000-0001-7719-7030
Ravi GuptaCollege of General Education, Kookmin University, Seoul 02707, Republic of Korea.ORCID 0000-0001-5242-9528
Cheol Woo MinDepartment of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea.ORCID 0000-0001-5966-6315
Sun Tae KimDepartment of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea.
Ai KimuraDepartment of Sport Sciences, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Ichiro TakasakiDepartment of Pharmacology, Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.ORCID 0000-0002-6083-1119
Motohide HoriDepartment of Oral Biology, Graduate School of Dentistry, Tohoku University, Sendai 980-8575, Japan.
Seiji ShiodaDepartment of Functional Morphology, Shonan University of Medical Sciences, 16-48 Kamishinano, Totsuka-ku, Yokohama 244-0806, Japan.
Randeep RakwalInstitute of Health and Sport Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8574, Japan.ORCID 0000-0003-0559-8577
Hoshi University · JPPusan National University · KRShonan University of Medical Sciences · JPKookmin University · KRTohoku University · JPUniversity of Toyama · JPUniversity of Tsukuba · JP

Funding

Japan Society for the Promotion of Science JP20K20392, JP22H03178
6 · The paper itself

Abstract

The study aimed to understand mechanism/s of neuronal outgrowth in the rat adrenal-derived pheochromocytoma cell line (PC12) under pituitary adenylate cyclase-activating polypeptide (PACAP) treatment. Neurite projection elongation was suggested to be mediated via Pac1 receptor-mediated dephosphorylation of CRMP2, where GSK-3β, CDK5, and Rho/ROCK dephosphorylated CRMP2 within 3 h after addition of PACAP, but the dephosphorylation of CRMP2 by PACAP remained unclear. Thus, we attempted to identify the early factors in PACAP-induced neurite projection elongation via omics-based transcriptomic (whole genome DNA microarray) and proteomic (TMT-labeled liquid chromatography-tandem mass spectrometry) analyses of gene and protein expression profiles from 5-120 min after PACAP addition. The results revealed a number of key regulators involved in neurite outgrowth, including known ones, called 'Initial Early Factors', e.g., genes

Indexed as

Phosphatidylinositol 3-KinasesPituitary Adenylate Cyclase-Activating PolypeptideAnimalsGlycogen Synthase Kinase 3 betaNeuronal OutgrowthOligonucleotide Array Sequence AnalysisPC12 CellsProteomicsRatsReceptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type IGlycogen Synthase Kinase 3 betaPhosphatidylinositol 3-KinasesPituitary Adenylate Cyclase-Activating PolypeptideReceptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type IbioinformaticsCRMP2DNA microarraygenomeLC-MS/MSneuronal differentiationPACAP38PC12proteome

Identifiers

PMID36835581
PMCPMC9964364
OpenAlexW4321377867

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.