Evidence map›Paper›PMID 36834842›Full record

ArticleInternational journal of molecular sciences2023

Characterizing and Quenching Autofluorescence in Fixed Mouse Adrenal Cortex Tissue.

Nawar Sakr, Olga Glazova, Liudmila Shevkova, Nikita Onyanov, Samira Kaziakhmedova, Alena Shilova, Maria V Vorontsova, Pavel Volchkov

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Nawar SakrEndocrinology Research Centre, Moscow 117292, Russia.ORCID 0000-0003-0768-4401
Olga GlazovaEndocrinology Research Centre, Moscow 117292, Russia.ORCID 0000-0001-7199-1866
Liudmila ShevkovaEndocrinology Research Centre, Moscow 117292, Russia.
Nikita OnyanovGenome Engineering Lab, Moscow Institute of Physics and Technology, Dolgoprudniy 141700, Russia.
Samira KaziakhmedovaGenome Engineering Lab, Moscow Institute of Physics and Technology, Dolgoprudniy 141700, Russia.
Alena ShilovaFaculty of Medicine, M.V. Lomonosov Moscow State University, 27-1, Lomonosovsky Prospect, Moscow 117192, Russia.
Maria V VorontsovaEndocrinology Research Centre, Moscow 117292, Russia.ORCID 0000-0002-9124-294X
Pavel VolchkovEndocrinology Research Centre, Moscow 117292, Russia.ORCID 0000-0001-9794-6297
Moscow Institute of Physics and Technology · RULomonosov Moscow State University · RU

Funding

Ministry of Science and Higher Education of the Russian Federation 075-15-2020-784
6 · The paper itself

Abstract

Tissue autofluorescence of fixed tissue sections is a major concern of fluorescence microscopy. The adrenal cortex emits intense intrinsic fluorescence that interferes with signals from fluorescent labels, resulting in poor-quality images and complicating data analysis. We used confocal scanning laser microscopy imaging and lambda scanning to characterize the mouse adrenal cortex autofluorescence. We evaluated the efficacy of tissue treatment methods in reducing the intensity of the observed autofluorescence, such as trypan blue, copper sulfate, ammonia/ethanol, Sudan Black B, TrueVIEW

Indexed as

Adrenal CortexLipofuscinAnimalsColoring AgentsFluorescenceIndicators and ReagentsMiceMicroscopy, ConfocalMicroscopy, FluorescenceTrypan BlueColoring AgentsIndicators and ReagentsLipofuscinTrypan Blueadrenal cortexautofluorescenceconfocal laser scanning microscopyfluorescence microscopyimmunofluorescence

Identifiers

PMID36834842
PMCPMC9968082
OpenAlexW4319761873

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.