ArticleMolecular and cellular biology1987
Specificity of RNA maturation pathways: RNAs transcribed by RNA polymerase III are not substrates for splicing or polyadenylation.
Article in Molecular and cellular biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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Who cites it
47 citing papers in PubMed, 115 citations in OpenAlex.
- RNA Polymerase II Activity of Type 3 Pol III Promoters.Molecular therapy. Nucleic acids · 2018Article
- Beyond Transcription: Roles of Transcription Factors in Pre-mRNA Splicing.Chemical reviews · 2018Review
- Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex.Nature chemical biology · 2017Article
- FUS functions in coupling transcription to splicing by mediating an interaction between RNAP II and U1 snRNP.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Coupling mRNA processing with transcription in time and space.Nature reviews. Genetics · 2014Review
- CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation.RNA biology · 2014Article
- Chromatin and splicing.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Co-transcriptional regulation of alternative pre-mRNA splicing.Biochimica et biophysica acta · 2012Review
- XPB, a subunit of TFIIH, is a target of the natural product triptolide.Nature chemical biology · 2011Article
- Review
- "Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions.Molecular cell · 2009Review
- The Carboxyl-terminal Domain of RNA Polymerase II Is Not Sufficient to Enhance the Efficiency of Pre-mRNA Capping or Splicing in the Context of a Different Polymerase.The Journal of biological chemistry · 2009Article
- Transcripts synthesized by RNA polymerase III can be polyadenylated in an AAUAAA-dependent manner.RNA (New York, N.Y.) · 2008Article
- Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing.American journal of human genetics · 2008Article
- RNA polymerase III transcripts and the PTB protein are essential for the integrity of the perinucleolar compartment.Molecular biology of the cell · 2003Article
- A Tetrahymena thermophila ribozyme-based indicator gene to detect transposition of marked retroelements in mammalian cells.Nucleic acids research · 2002Article
- T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export.RNA (New York, N.Y.) · 2002Article
- Messenger RNAs that are not synthesized by RNA polymerase II can be 3' end cleaved and polyadenylated.EMBO reports · 2000Article
- Assembly and functional organization of the nucleolus: ultrastructural analysis of Saccharomyces cerevisiae mutants.Molecular biology of the cell · 2000Article
- Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.Microbiology and molecular biology reviews : MMBR · 1999Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To analyze the specificity of RNA processing reactions, we constructed hybrid genes containing RNA polymerase III promoters fused to sequences that are normally transcribed by polymerase II and assessed their transcripts following transfection into human 293 cells. Transcripts derived from these chimeric constructs were analyzed by using a combined RNase H and S1 nuclease assay to test whether RNAs containing consensus 5' and 3' splicing signals could be efficiently spliced in intact cells, even though they were transcribed by RNA polymerase III. We found that polymerase III-derived RNAs are not substrates for splicing. Similarly, we were not able to detect poly(A)+ RNAs derived from genes that contained a polymerase III promoter linked to sequences that were necessary and sufficient to direct 3'-end cleavage and polyadenylation when transcribed by RNA polymerase II. Our findings are consistent with the view that in vivo splicing and polyadenylation pathways are obligatorily coupled to transcription by RNA polymerase II.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.