Evidence map›Paper›PMID 36832059›Full record

ArticleBiosensors2023

A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging.

Heng Tang, Junran Peng, Xin Jiang, Shuang Peng, Fang Wang, Xiaocheng Weng, Xiang Zhou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. Imaging methods to monitor and quantify cell differentiation.Frontiers in cell and developmental biology · 2025
    Review
  4. CRISPR/Cas-Based Techniques for Live-Cell Imaging and Bioanalysis.International journal of molecular sciences · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Heng TangDepartment of Clinical Laboratory, Center for Gene Diagnosis, Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Junran PengDepartment of Clinical Laboratory, Center for Gene Diagnosis, Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Xin JiangDepartment of Clinical Laboratory, Center for Gene Diagnosis, Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Shuang PengDepartment of Clinical Laboratory, Center for Gene Diagnosis, Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Fang WangSchool of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Xiaocheng WengDepartment of Clinical Laboratory, Center for Gene Diagnosis, Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Xiang ZhouDepartment of Clinical Laboratory, Center for Gene Diagnosis, Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Wuhan University · CNZhongnan Hospital of Wuhan University · CN

Funding

National Natural Science Foundation of China 21721005National Natural Science Foundation of China 22177087National Natural Science Foundation of China 22277094National Natural Science Foundation of China 91753201National Natural Science Foundation of China 91940000National Natural Science Foundation of China 92253202
6 · The paper itself

Abstract

We reported on an efficient RNA imaging strategy based on a CRISPR-Cas and Tat peptide with a fluorescent RNA aptamer (TRAP-tag). Using modified CRISPR-Cas RNA hairpin binding proteins fused with a Tat peptide array that recruits modified RNA aptamers, this simple and sensitive strategy is capable of visualizing endogenous RNA in cells with high precision and efficiency. In addition, the modular design of the CRISPR-TRAP-tag facilitates the substitution of sgRNAs, RNA hairpin binding proteins, and aptamers in order to optimize imaging quality and live cell affinity. With CRISPR-TRAP-tag, exogenous

Indexed as

Aptamers, NucleotideRNACRISPR-Cas SystemsDiagnostic ImagingPeptidesAptamers, NucleotidePeptidesRNACRISPRRNA aptamerRNA imagingTat peptide

Identifiers

PMID36832059
PMCPMC9954185
OpenAlexW4321374035

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.