Evidence map›Paper›PMID 36832019›Full record

ArticleBiosensors2023

Fluholoscopy-Compact and Simple Platform Combining Fluorescence and Holographic Microscopy.

David Alonso, Javier Garcia, Vicente Micó

Abstract read
In one paragraph

Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

David AlonsoDepartamento de Óptica y de Optometría y Ciencias de la Visión, Facultad de Física, Universidad de Valencia, C/Doctor Moliner 50, 46100 Burjassot, Spain.ORCID 0000-0002-7804-8720
Javier GarciaDepartamento de Óptica y de Optometría y Ciencias de la Visión, Facultad de Física, Universidad de Valencia, C/Doctor Moliner 50, 46100 Burjassot, Spain.
Vicente MicóDepartamento de Óptica y de Optometría y Ciencias de la Visión, Facultad de Física, Universidad de Valencia, C/Doctor Moliner 50, 46100 Burjassot, Spain.ORCID 0000-0001-9457-1960

Funding

Ministerio de ciencia e innovacion (MCIN/AEI/10.13039/501100011033) PID2020-120056GB-C21
6 · The paper itself

Abstract

The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences as it permits the analysis of complementary properties of the target sample. Here, we report on an extremely simple, cost-effective, and compact microscope platform for achieving simultaneous fluorescence and quantitative phase imaging modes with the capability of working in a single snapshot. It is based on the use of a single illumination wavelength to both excite the sample's fluorescence and provide coherent illumination for phase imaging. After passing the microscope layout, the two imaging paths are separated using a bandpass filter, and the two imaging modes are simultaneously obtained using two digital cameras. We first present calibration and analysis of both fluorescence and phase imaging modalities working independently and, later on, experimental validation for the proposed common-path dual-mode imaging platform considering static (resolution test targets, fluorescent micro-beads, and water-suspended lab-made cultures) as well as dynamic (flowing fluorescent beads, human sperm cells, and live specimens from lab-made cultures) samples.

Indexed as

HolographyMicroscopyCalibrationHumansLightingMaleSemenfluorescence imagingGabor holographymultimodal imagingmultiplexed microscopyquantitative phase imaging

Identifiers

PMID36832019
PMCPMC9954010

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.