Evidence map›Paper›PMID 36831965›Full record

ArticleBiosensors2023

A Monoclonal Antibody-Based Immunochromatographic Test Strip and Its Application in the Rapid Detection of Cucumber Green Mottle Mosaic Virus.

Zichen Zhao, Yanli Tian, Chang Xu, Yuanfei Xing, Lili Yang, Guoliang Qian, Xiude Hua, Weirong Gong, Baishi Hu, Limin Wang

Open access · goldAbstract read
In one paragraph

Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Zichen ZhaoDepartment of Phytopathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Yanli TianDepartment of Phytopathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Chang XuDepartment of Phytopathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Yuanfei XingDepartment of Phytopathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Lili YangDepartment of Phytopathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Guoliang QianDepartment of Phytopathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0000-0003-3577-3241
Xiude HuaDepartment of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0000-0003-0557-1892
Weirong GongPlant Protection and Quarantine Station of Jiangsu Province, Nanjing 210036, China.
Baishi HuDepartment of Phytopathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Limin WangDepartment of Phytopathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Nanjing Agricultural University · CNInstitute of Plant Protection · CN

Funding

National Key Research and Development Program of China 2022YFD1400203National Natural Science Foundation of China 31772231Science and technology project of Shanxi Branch of China National Tobacco Corporation KJ-2022-04
6 · The paper itself

Abstract

Two specific monoclonal antibodies (mAbs) were screened, and an immunochromatographic strip (ICS) test for rapid and specific detection of cucumber green mottle mosaic virus (CGMMV) was developed. The coat protein of CGMMV was heterologously expressed as an immunogen, and specific capture mAb 2C9 and the detection mAb 4D4 were screened by an uncompetitive immunoassay. The test and control lines on the nitrocellulose membrane were coated with the purified 2C9 and a goat anti-mouse IgG, respectively, and a nanogold probe combined with 4D4 was applied to the conjugate pad. Using these mAbs, a rapid and sensitive ICS was developed. Within the sandwich mode of 2C9-CGMMV-4D4, the test line showed a corresponding positive relationship with CGMMV in infected samples. The ICS test had a detection limit of 1:5000 (

Indexed as

Antibodies, MonoclonalTobamovirusImmunologic TestsPlant DiseasesAntibodies, Monoclonalcucumber green mottle mosaic virusimmunochromatographic stripmonoclonal antibodiessandwich

Identifiers

PMID36831965
PMCPMC9953337
OpenAlexW4318476601

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.