Evidence map›Paper›PMID 36831948›Full record

ArticleBiosensors2023

SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering.

Markéta Benešová, Silvie Bernatová, Filip Mika, Zuzana Pokorná, Jan Ježek, Martin Šiler, Ota Samek, Filip Růžička, Katarina Rebrošová, Pavel Zemánek and 1 more

Open access · goldAbstract read
In one paragraph

Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Markéta BenešováInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
Silvie BernatováInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
Filip MikaInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.ORCID 0000-0001-8370-2929
Zuzana PokornáInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.ORCID 0000-0002-2413-6944
Jan JežekInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
Martin ŠilerInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.ORCID 0000-0001-9040-4735
Ota SamekInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
Filip RůžičkaDepartment of Microbiology, Faculty of Medicine of Masaryk University and St. Anne's University Hospital, Pekařská 53, 656 91 Brno, Czech Republic.
Katarina RebrošováDepartment of Microbiology, Faculty of Medicine of Masaryk University and St. Anne's University Hospital, Pekařská 53, 656 91 Brno, Czech Republic.ORCID 0000-0002-6577-1864
Pavel ZemánekInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.
Zdeněk PilátInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic.ORCID 0000-0001-9259-8393
Czech Academy of Sciences, Institute of Scientific Instruments · CZMasaryk University · CZ

Funding

Czech Academy of Sciences MSM100652101Czech Academy of Sciences RVO:68081731Ministry of Health of the Czech Republic NU21-05-00341
6 · The paper itself

Abstract

Efficient separation and sensitive identification of pathogenic bacterial strains is essential for a prosperous modern society, with direct applications in medical diagnostics, drug discovery, biodefense, and food safety. We developed a fast and reliable method for antibody-based selective immobilization of bacteria from suspension onto a gold-plated glass surface, followed by detection using strain-specific antibodies linked to gold nanoparticles decorated with a reporter molecule. The reporter molecules are subsequently detected by surface-enhanced Raman spectroscopy (SERS). Such a multi-functionalized nanoparticle is called a SERS-tag. The presented procedure uses widely accessible and cheap materials for manufacturing and functionalization of the nanoparticles and the immobilization surfaces. Here, we exemplify the use of the produced SERS-tags for sensitive single-cell detection of opportunistic pathogen

Indexed as

Metal NanoparticlesSpectrum Analysis, RamanAntibodiesEscherichia coliGoldStaphylococcus aureusAntibodiesGoldEscherichia colisandwich immunoassaySERS-tagsingle-cell detection

Identifiers

PMID36831948
PMCPMC9954015
OpenAlexW4317932675

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.