Evidence map›Paper›PMID 36831926›Full record

ReviewBiosensors2023

Enzyme-Assisted Nucleic Acid Amplification in Molecular Diagnosis: A Review.

Meiling Wang, Hongna Liu, Jie Ren, Yunqi Huang, Yan Deng, Yuan Liu, Zhu Chen, Franklin Wang-Ngai Chow, Polly Hang-Mei Leung, Song Li

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Meiling WangHunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.
Hongna LiuHunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.
Jie RenHunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.
Yunqi HuangHunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.
Yan DengHunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.
Yuan LiuHengyang Medical School, University of South China, Hengyang 421001, China.
Zhu ChenHunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.
Franklin Wang-Ngai ChowDepartment of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong 999077, China.ORCID 0000-0003-1275-2464
Polly Hang-Mei LeungDepartment of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong 999077, China.ORCID 0000-0003-2406-4773
Song LiHunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.

Funding

Hunan Key R&D Projects 2021SK2003Hunan Key R&D Projects 2022SK2115Hunan Provincial Natural Science Foundation of China 2019JJ50122the National Key R&D Program of China 2019YFE0191000the National Key R&D Program of China 2021YFE0191400the National Key R&D Program of China 2021YFE0200400the National Natural Science Foundation of China 61871180the National Natural Science Foundation of China 61971187
6 · The paper itself

Abstract

Infectious diseases and tumors have become the biggest medical challenges in the 21st century. They are driven by multiple factors such as population growth, aging, climate change, genetic predispositions and more. Nucleic acid amplification technologies (NAATs) are used for rapid and accurate diagnostic testing, providing critical information in order to facilitate better follow-up treatment and prognosis. NAATs are widely used due their high sensitivity, specificity, rapid amplification and detection. It should be noted that different NAATs can be selected according to different environments and research fields; for example, isothermal amplification with a simple operation can be preferred in developing countries or resource-poor areas. In the field of translational medicine, CRISPR has shown great prospects. The core component of NAAT lies in the activity of different enzymes. As the most critical material of nucleic acid amplification, the key role of the enzyme is self-evident, playing the upmost important role in molecular diagnosis. In this review, several common enzymes used in NAATs are compared and described in detail. Furthermore, we summarize both the advances and common issues of NAATs in clinical application.

Indexed as

Communicable DiseasesNucleic AcidsHumansNucleic Acid Amplification TechniquesNucleic Acidsclinical applicationCRISPR-Casenzymesisothermal amplificationnucleic acid amplification technologypolymerase chain reaction

Identifiers

PMID36831926
PMCPMC9953907

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.