Evidence map›Paper›PMID 36829594›Full record

ReviewBiology2023

Single-Cell Labeling Strategies to Dissect Neuronal Structures and Local Functions.

Keigo Kohara, Masayoshi Okada

Abstract readReview
In one paragraph

Review in Biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Keigo KoharaKMU Biobank Center, Institute of Biomedical Science, Kansai Medical University, Hirakata 573-1010, Japan.ORCID 0000-0003-1521-184X
Masayoshi OkadaDepartment of Medical Life Science, College of Life Science, Kurashiki University of Science and the Arts, Kurashiki 712-8505, Japan.ORCID 0000-0001-8515-7143

Funding

Kaken Pharmaceutical (Japan) 19K22583
6 · The paper itself

Abstract

The brain network consists of ten billion neurons and is the most complex structure in the universe. Understanding the structure of complex brain networks and neuronal functions is one of the main goals of modern neuroscience. Since the seminal invention of Golgi staining, single-cell labeling methods have been among the most potent approaches for dissecting neuronal structures and neural circuits. Furthermore, the development of sparse single-cell transgenic methods has enabled single-cell gene knockout studies to examine the local functions of various genes in neural circuits and synapses. Here, we review non-transgenic single-cell labeling methods and recent advances in transgenic strategies for sparse single neuronal labeling. These methods and strategies will fundamentally contribute to the understanding of brain structure and function.

Indexed as

cre recombinasefluorescent proteinmorphologyneural circuitneuronneuronal activitysingle-cell activity manipulating analysissingle-cell gene knockoutsingle-cell labeling methodssingle-cell silencing and activationsynapse

Identifiers

PMID36829594
PMCPMC9953318

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.