ArticleResearch square2023
Shuttle Peptide Delivers Base Editor RNPs to Rhesus Monkey Airway Epithelial Cells
Katarina Kulhankova, Soumba Traore, Xue Cheng, Hadrien Benk-Fortin, Stéphanie Hallée, Mario Harvey, Joannie Roberge, Frédéric Couture, Thomas Gross, Gregory Newby and 4 more
Open access · greenAbstract readPreprint
In one paragraphArticle in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed, 5 citations in OpenAlex.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
14 authors at 4 institutions in 2 countries.
Katarina KulhankovaUniversity of Iowa.
Soumba TraoreUniversity of Iowa.
Xue ChengFeldan Therapeutics.
Hadrien Benk-FortinFeldan Therapeutics.
Stéphanie HalléeFeldan Therapeutics.
Mario HarveyFeldan Therapeutics.
Joannie RobergeFeldan Therapeutics.
Frédéric CoutureTransBIOTech.
Gregory NewbyBroad Institute.
David LiuBroad Institute.
Alice TarantalUniversity of California - Davis.
David GuayFeldan Therapeutics.
Feldan (Canada) · CAUniversity of Iowa · USBroad Institute · USUniversity of California, Davis · US
Funding
National Institute on Aging (NIA) ColonyP51OD011107 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Simon J. Atkinson · 2012 to 2026
$191.5MVector Core-Core 2P30DK054759 · NIDDK · UNIVERSITY OF IOWA · PI Amy Leanne Ryan · 1998 to 2026
$30.5MDissemination and Coordinating Center for the SCGE ConsortiumU24HG010423 · NHGRI · MEDICAL COLLEGE OF WISCONSIN · PI DWINELL, MELINDA R · 2019 to 2023
$15.0MSCGE Comparative Studies SupplementU42OD027094 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI TARANTAL, ALICE F · 2019 to 2023
$14.2MPathology CoreP01HL152960 · NHLBI · UNIVERSITY OF IOWA · PI WELSH, MICHAEL J. · 2020 to 2024
$11.6MSCGE Disease Models Studies Supplement: Repair of a CFTR Nonsense Mutation Using Adenine Base EditingUH3HL147366 · NHLBI · UNIVERSITY OF IOWA · PI MCCRAY, PAUL B · 2021 to 2022
$2.3MDelivery Technologies for In Vivo Genome EditingUG3AI150551 · NIAID · BETH ISRAEL DEACONESS MEDICAL CENTER · PI CHAIKOF, ELLIOT · 2019 to 2021
$2.3MPET/CT for Nonhuman Primate ImagingS10RR025063 · NCRR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI TARANTAL, ALICE F · 2011 to 2011
$2.1MExpanding the Scope of Base EditingU01AI142756 · NIAID · BROAD INSTITUTE, INC. · PI LIU, DAVID R · 2018 to 2022
$2.1MDelivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic PeptidesUG3HL147366 · NHLBI · UNIVERSITY OF IOWA · PI MCCRAY, PAUL B · 2018 to 2020
$2.1MminiEXPLORER-II for Total-Body PET Translational ImagingS10OD028713 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI TARANTAL, ALICE F · 2020 to 2020
$1.9MIn vivo precision genome editing to correct genetic diseaseR00HL163805 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI NEWBY, GREGORY A. · 2023 to 2025
$747kNCRR NIH HHS S10 RR025063NHGRI NIH HHS U24 HG010423NHLBI NIH HHS K99 HL163805NHLBI NIH HHS P01 HL152960NHLBI NIH HHS R00 HL163805NHLBI NIH HHS UG3 HL147366NHLBI NIH HHS UH3 HL147366NIAID NIH HHS U01 AI142756NIAID NIH HHS UG3 AI150551NIDDK NIH HHS P30 DK054759NIH HHS P51 OD011107NIH HHS S10 OD016261NIH HHS S10 OD028713NIH HHS U42 OD027094
6 · The paper itselfAbstract
Gene editing strategies for cystic fibrosis are challenged by the complex barrier properties of airway epithelia. We previously reported that the amphiphilic S10 shuttle peptide non-covalently combined with CRISPR-associated (Cas) ribonucleoprotein (RNP) enabled editing of human and mouse airway epithelial cells. Here, to improve base editor RNP delivery, we optimized S10 to derive the S315 peptide. Following intratracheal aerosol of Cy5-labeled peptide cargo in rhesus macaques, we confirmed delivery throughout the respiratory tract. Subsequently, we targeted
Indexed as
adenine base editorcystic fibrosisribonucleoprotein
Identifiers
PMID36824928
PMCPMC9949254
OpenAlexW4321185950
What OpenQuestion holds
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LicenceCC BY
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