Evidence map›Paper›PMID 36824829›Full record

ArticlebioRxiv : the preprint server for biology2023

Overcoming resolution loss due to thermal magnetic field fluctuations from phase plates in transmission electron microscopy.

Jeremy J Axelrod, Petar N Petrov, Jessie T Zhang, Jonathan Remis, Bart Buijsse, Robert M Glaeser, Holger Mȕller

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Jeremy J AxelrodDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA.
Petar N PetrovDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA.
Jessie T ZhangDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA.
Jonathan RemisDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA.
Bart BuijsseThermo Fisher Scientific, Achtseweg Noord 5, 5651 GG, Eindhoven, the Netherlands.
Robert M GlaeserLawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA.
Holger MȕllerDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA.
Lawrence Berkeley National Laboratory · USThermo Fisher Scientific (Netherlands) · NLUniversity of California, Berkeley · US

Funding

Development of Laser-Based Phase Contrast for Biological Electron MicroscopyR01GM126011 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI MUELLER, HOLGER · 2017 to 2024
$3.9M
Investigating MHC-I downregulation by HIV-1 Nef using cryo-electron tomography with laser-based phase contrastF32GM149186 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI PETROV, PETAR NIKOLOV · 2023 to 2023
$70k
NIGMS NIH HHS F32 GM149186NIGMS NIH HHS R01 GM126011
6 · The paper itself

Abstract

We identify thermal magnetic field fluctuations, caused by thermal electron motion ("Johnson noise") in electrically conductive materials, as a potential resolution limit in transmission electron microscopy with a phase plate. Specifically, resolution loss can occur if the electron diffraction pattern is magnified to extend phase contrast to lower spatial frequencies, and if conductive materials are placed too close to the electron beam. While our initial implementation of a laser phase plate (LPP) was significantly affected by these factors, a redesign eliminated the problem and brought the performance close to the expected level. The resolution now appears to be limited by residual Johnson noise arising from the electron beam liner tube in the region of the LPP, together with the chromatic aberration of the relay optics. These two factors can be addressed during future development of the LPP.

Identifiers

PMID36824829
PMCPMC9949102
OpenAlexW4320481856

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.